|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2004-10-11
|
|
pubmed:abstractText |
The novel basic, heat-stable tubulin polymerization promoting protein TPPP/p25 is associated with microtubules in vitro and can induce the formation of aberrant microtubule assemblies. We show by 1H-NMR spectroscopy that TPPP/p25 is natively unfolded. Antisera against peptide 186GKGKAGRVDLVDESG200NH2 (186-200) are highly specific to TPPP/p25. Immunohistochemistry and confocal microscopy demonstrates that TPPP/p25 is enriched in filamentous alpha-synuclein bearing Lewy bodies of Parkinson's (PD) and diffuse Lewy body disease (DLBD), as well as glial inclusions of multiple system atrophy (MSA). There is a correlation between TPPP/p25 and alpha-synuclein immunoreactivity in Western blot. In contrast, TPPP/p25 is not associated with abnormally phosphorylated tau in various inclusions of Pick's disease (PiD), progressive supranuclear palsy (PSP), and corticobasal degeneration (CBD). However, electron microscopy confirms clusters of TPPP/p25 immunoreactivity along filaments of unstructured but not compact neurofibrillary tangles in Alzheimer's disease (AD). TPPP/p25 seems to be a novel marker of alpha-synucleinopathies.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0969-9961
|
|
pubmed:author |
pubmed-author:BotondGergoG,
pubmed-author:BudkaHerbertH,
pubmed-author:ErdeiAnnaA,
pubmed-author:GelpiEllenE,
pubmed-author:HudeczFerencF,
pubmed-author:JensenPoul HenningPH,
pubmed-author:KovácsGábor GGG,
pubmed-author:KovácsJánosJ,
pubmed-author:LászlóLajosL,
pubmed-author:LehotzkyAttilaA,
pubmed-author:LinderssonEvoE,
pubmed-author:MezoGáborG,
pubmed-author:MolnárTamásT,
pubmed-author:OvádiJuditJ,
pubmed-author:PerczelAndrásA,
pubmed-author:TiriánLászlóL
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
17
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
155-62
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:15474353-Aged,
pubmed-meshheading:15474353-Animals,
pubmed-meshheading:15474353-Biological Markers,
pubmed-meshheading:15474353-Blotting, Western,
pubmed-meshheading:15474353-Brain,
pubmed-meshheading:15474353-Cattle,
pubmed-meshheading:15474353-Female,
pubmed-meshheading:15474353-Humans,
pubmed-meshheading:15474353-Immune Sera,
pubmed-meshheading:15474353-Immunohistochemistry,
pubmed-meshheading:15474353-Magnetic Resonance Spectroscopy,
pubmed-meshheading:15474353-Male,
pubmed-meshheading:15474353-Microscopy, Electron,
pubmed-meshheading:15474353-Middle Aged,
pubmed-meshheading:15474353-Nerve Tissue Proteins,
pubmed-meshheading:15474353-Neurodegenerative Diseases,
pubmed-meshheading:15474353-Protein Folding,
pubmed-meshheading:15474353-Synucleins,
pubmed-meshheading:15474353-alpha-Synuclein
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Natively unfolded tubulin polymerization promoting protein TPPP/p25 is a common marker of alpha-synucleinopathies.
|
|
pubmed:affiliation |
Institute of Neurology, Medical University of Vienna, A-1097 Vienna, Austria.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
