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rdf:type |
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lifeskim:mentions |
umls-concept:C0017337,
umls-concept:C0042567,
umls-concept:C0043457,
umls-concept:C0314603,
umls-concept:C0521378,
umls-concept:C0542341,
umls-concept:C0851285,
umls-concept:C1417951,
umls-concept:C1527148,
umls-concept:C1704259,
umls-concept:C1705987
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pubmed:issue |
2
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pubmed:dateCreated |
2004-9-23
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pubmed:abstractText |
Targeted disruption of the onecut transcription factor, hnf-6, alters mammalian biliary system development. We have identified a related zebrafish cDNA expressed in the developing liver that is a functional ortholog of mammalian hnf-6. Antisense-mediated knockdown of zebrafish hnf-6 perturbs development of the intrahepatic biliary system. Knockdown of zebrafish hnf-6 alters expression of vhnf1 and the zebrafish orthologs of other mammalian genes regulated by hnf-6. Coinjection of mRNA encoding zebrafish vhnf1 rescues the biliary phenotype of hnf-6 morphants. These experiments strongly suggest that hnf-6 and vhnf1 function within an evolutionarily conserved pathway that regulates biliary development. Forced expression of either hnf-6 or vhnf1 also produces biliary phenotypes. Altered bile duct development in both loss- and gain-of-function experiments suggests that zebrafish biliary cells are sensitive to the dosage of hnf-6-mediated gene transcription.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HNF1B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 6,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/hnf1ba protein, zebrafish
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0012-1606
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
245-59
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15385156-Amino Acid Sequence,
pubmed-meshheading:15385156-Animals,
pubmed-meshheading:15385156-Base Sequence,
pubmed-meshheading:15385156-Bile Ducts,
pubmed-meshheading:15385156-Biological Evolution,
pubmed-meshheading:15385156-DNA-Binding Proteins,
pubmed-meshheading:15385156-Gene Expression Regulation, Developmental,
pubmed-meshheading:15385156-Hepatocyte Nuclear Factor 1-beta,
pubmed-meshheading:15385156-Hepatocyte Nuclear Factor 6,
pubmed-meshheading:15385156-Homeodomain Proteins,
pubmed-meshheading:15385156-Humans,
pubmed-meshheading:15385156-In Situ Hybridization,
pubmed-meshheading:15385156-Lipids,
pubmed-meshheading:15385156-Liver,
pubmed-meshheading:15385156-Molecular Sequence Data,
pubmed-meshheading:15385156-Oligonucleotides, Antisense,
pubmed-meshheading:15385156-Pancreas,
pubmed-meshheading:15385156-RNA, Messenger,
pubmed-meshheading:15385156-Sequence Alignment,
pubmed-meshheading:15385156-Trans-Activators,
pubmed-meshheading:15385156-Transcription Factors,
pubmed-meshheading:15385156-Zebrafish,
pubmed-meshheading:15385156-Zebrafish Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
The zebrafish onecut gene hnf-6 functions in an evolutionarily conserved genetic pathway that regulates vertebrate biliary development.
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pubmed:affiliation |
Division of Gastroenterology and Nutrition, Children's Hospital of Philadelphia and Department of Pediatrics, University of Pennsylvania School of Medicine, 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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