Source:http://linkedlifedata.com/resource/pubmed/id/15358679
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
2004-11-16
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| pubmed:abstractText |
In this study, we investigated the mechanisms through which norepinephrine (NE) regulates MAPK phosphatase-1 (MKP-1) expression in rat pinealocytes. Stimulation with NE (a mixed alpha- and beta-adrenergic agonist) caused a rapid increase in MKP-1 mRNA and protein that peaked around 1 h post stimulation, and the response was sustained for at least 4 h. Selective activation of beta-adrenergic receptors with isoproterenol for 1 h caused a similar increase in MKP-1 mRNA and protein as observed with NE, but at 3 h, the isoproterenol response was much lower relative to NE. In contrast, selective activation of alpha-adrenergic receptors caused only small increases in MKP-1 mRNA and protein and appeared to function primarily in prolonging the beta-adrenergic-stimulated responses. In NE-stimulated pinealocytes, blockade of beta-adrenergic receptors caused a rapid reduction in MKP-1 mRNA, but it had a minimal effect on MKP-1 protein. In contrast, blockade of alpha-adrenergic receptors specifically reduced NE-induced MKP-1 protein but not mRNA. At the postreceptor level, treatment with dibutyryl cAMP caused parallel increases in MKP-1 mRNA and protein. However, treatment with a protein kinase C activator caused a significant increase in MKP-1 protein but had little effect on MKP-1 mRNA. Together, these results suggest that, in rat pinealocytes, NE activates the beta-adrenergic receptor --> protein kinase A pathway to induce transcription and translation of MKP-1 expression and the alpha-adrenergic receptor --> protein kinase C pathway to prolong the stimulated responses through increased stability of the MKP-1 protein.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Dual Specificity Phosphatase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Dusp1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Phosphatase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta,
http://linkedlifedata.com/resource/pubmed/chemical/Sympathomimetics
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0013-7227
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
145
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5723-33
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15358679-Animals,
pubmed-meshheading:15358679-Cell Cycle Proteins,
pubmed-meshheading:15358679-Cells, Cultured,
pubmed-meshheading:15358679-Dual Specificity Phosphatase 1,
pubmed-meshheading:15358679-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:15358679-Immediate-Early Proteins,
pubmed-meshheading:15358679-MAP Kinase Signaling System,
pubmed-meshheading:15358679-Male,
pubmed-meshheading:15358679-Norepinephrine,
pubmed-meshheading:15358679-Phosphoprotein Phosphatases,
pubmed-meshheading:15358679-Pineal Gland,
pubmed-meshheading:15358679-Protein Phosphatase 1,
pubmed-meshheading:15358679-Protein Tyrosine Phosphatases,
pubmed-meshheading:15358679-RNA, Messenger,
pubmed-meshheading:15358679-Rats,
pubmed-meshheading:15358679-Rats, Sprague-Dawley,
pubmed-meshheading:15358679-Receptors, Adrenergic, alpha,
pubmed-meshheading:15358679-Receptors, Adrenergic, beta,
pubmed-meshheading:15358679-Sympathomimetics
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| pubmed:year |
2004
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| pubmed:articleTitle |
Norepinephrine induction of mitogen-activated protein kinase phosphatase-1 expression in rat pinealocytes: distinct roles of alpha- and beta-adrenergic receptors.
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| pubmed:affiliation |
Department of Physiology, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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