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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1992-7-2
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pubmed:abstractText |
In an attempt to identify the phenotype of the CD25+ cells previously demonstrated in late-phase allergic reactions in skin and nasal mucosa, and in the bronchial mucosa in atopic asthma we have investigated the co-expression of CD3 and CD25 using double immunofluorescent staining. Fluorescein isothiocyanate (FITC) conjugated anti-CD3 monoclonal antibody and a biotinylated anti-CD25 monoclonal antibody (which was later developed with Texas Red streptavidin) were applied simultaneously to cryostat sections or cytospins of freshly prepared samples. Skin and nasal biopsies were from antigen-induced late-phase reactions, and bronchial biopsies and cytospins of bronchoalveolar lavage cells were from subjects with moderate atopic asthma. Four to 14% of CD3+ cells in these tissues were CD25+ and 61-100% of CD25+ cells also expressed CD3. However, CD25 was also expressed on non-CD3+ cells, assumed to be non-T cells. These results were comparable to that observed with a positive control of a tuberculin-challenged skin biopsy. Thus the majority of cells expressing CD25 in various types of atopic allergic inflammation in man were T lymphocytes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-1673877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-1777835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-1830598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-1885772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-1918731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-1997656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2022726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2101599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2252260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2690708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2783333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2826636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2896958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2967330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-2974055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-3118989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-6408171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1534311-77279
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
659-63
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1534311-Antigens, CD,
pubmed-meshheading:1534311-Antigens, CD3,
pubmed-meshheading:1534311-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:1534311-Asthma,
pubmed-meshheading:1534311-Bronchi,
pubmed-meshheading:1534311-Fluorescent Antibody Technique,
pubmed-meshheading:1534311-Humans,
pubmed-meshheading:1534311-Hypersensitivity, Immediate,
pubmed-meshheading:1534311-Nasal Cavity,
pubmed-meshheading:1534311-Receptors, Antigen, T-Cell,
pubmed-meshheading:1534311-Receptors, Interleukin-2,
pubmed-meshheading:1534311-Skin
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pubmed:year |
1992
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pubmed:articleTitle |
Co-expression of CD25 and CD3 in atopic allergy and asthma.
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pubmed:affiliation |
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, U.K.
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pubmed:publicationType |
Journal Article
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