Linked Life Data

15246011

Source:http://linkedlifedata.com/resource/pubmed/id/15246011

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-7-12
pubmed:abstractText
A novel spectrophotometric assay for monitoring structural rearrangements of native low-density lipoproteins (LDL) is proposed. The approach is based on the analysis of the visible light absorbance maximum of lipoproteins at approximately 461 nm assigned to beta-carotene situated in the hydrophobic parts of LDL. It offers a direct method to study the surface-interior coupling of the lipoprotein particle under physiological conditions. The detected signal is intrinsic to LDL and responsible for the most of the beta-carotene signal from the whole plasma. The negligible interference of beta-carotene absorbance due to the high-density lipoproteins is experimentally verified. Since beta-carotene absorbance belongs to the visible spectral region, no spectral overlapping/artifacts in plasma are expected. The signal sensitivity has been studied through conformational changes of LDL induced by ionic strength, by temperature, and by ligand binding. The results of caffeine binding to LDL indicate that there could be only one dominant type of binding site for caffeine on LDL particles. It can be concluded that visible spectrum characteristics of beta-carotene molecules offer advantages in LDL ligand binding studies which can possibly be extended to monitor the interactions of LDL directly in plasma.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
331
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Analysis of beta-carotene absorbance for studying structural properties of human plasma low-density lipoproteins.
pubmed:affiliation
Ruer Boskovi? Institute, Bijenicka 54, 10000 Zagreb, Croatia. akrisko@irb.hr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't