| pubmed-article:15231699 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C0034833 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C0025543 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C0221099 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C1704241 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C0231491 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:15231699 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:15231699 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:15231699 | pubmed:dateCreated | 2004-9-17 | lld:pubmed |
| pubmed-article:15231699 | pubmed:abstractText | ADAMTS-1, a member of the A disintegrin and metalloproteinase family of proteases, is expressed in rodent follicles via progesterone receptor (PR)-dependent pathways. However, the functional relationship between ADAMTS-1 expression and PR has not been studied extensively in other species. In the present study, we investigated the time-dependent changes in ADAMTS-1 expression in cumulus cells of porcine cumulus-oocyte complexes (COCs), and the roles of ADAMTS-1 in cumulus expansion during in vitro maturation of oocytes. ADAMTS-1 message was not detected in cumulus cells at the time of collection from the follicles. In response to gonadotropins, ADAMTS-1 mRNA was dramatically up-regulated and reached a maximum at 20 h. The level of mature ADAMTS-1 protein increased in a time-dependent manner with a maximum level at 40 h. The induction of ADAMTS-1 mRNA and protein was significantly decreased by the addition of PR antagonist RU486 to the cultures. However, RU486 did not affect the expression of ADAMTS-4 or factors that had been reported to be required for COC expansion (TSG-6, versican, HA synthase-2). COCs cultured with FSH and LH for 40 h exhibited prominent cumulus expansion. The expansion was reduced significantly by the addition of either RU486 or Galardin, a broad-spectrum matrix metalloproteinase inhibitor. These results suggest that the expression and induction of ADAMTS-1 through receptor-mediated action of progesterone in cumulus cells is one of the essential requirements for gonadotropin-regulated cumulus expansion of porcine COCs. | lld:pubmed |
| pubmed-article:15231699 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15231699 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231699 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15231699 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:15231699 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:15231699 | pubmed:author | pubmed-author:MoriTakahideT | lld:pubmed |
| pubmed-article:15231699 | pubmed:author | pubmed-author:RichardsJoAnn... | lld:pubmed |
| pubmed-article:15231699 | pubmed:author | pubmed-author:NishiboriMasa... | lld:pubmed |
| pubmed-article:15231699 | pubmed:author | pubmed-author:ShimadaMasayu... | lld:pubmed |
| pubmed-article:15231699 | pubmed:author | pubmed-author:YamashitaYasu... | lld:pubmed |
| pubmed-article:15231699 | pubmed:author | pubmed-author:ItoJunyaJ | lld:pubmed |
| pubmed-article:15231699 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15231699 | pubmed:volume | 145 | lld:pubmed |
| pubmed-article:15231699 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15231699 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15231699 | pubmed:pagination | 4603-14 | lld:pubmed |
| pubmed-article:15231699 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:15231699 | pubmed:meshHeading | pubmed-meshheading:15231699... | lld:pubmed |
| pubmed-article:15231699 | pubmed:meshHeading | pubmed-meshheading:15231699... | lld:pubmed |
| pubmed-article:15231699 | pubmed:meshHeading | pubmed-meshheading:15231699... | lld:pubmed |
| pubmed-article:15231699 | pubmed:meshHeading | pubmed-meshheading:15231699... | lld:pubmed |
| pubmed-article:15231699 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15231699 | pubmed:articleTitle | Down-regulated expression of A disintegrin and metalloproteinase with thrombospondin-like repeats-1 by progesterone receptor antagonist is associated with impaired expansion of porcine cumulus-oocyte complexes. | lld:pubmed |
| pubmed-article:15231699 | pubmed:affiliation | Department of Applied Animal Science, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8528, Japan. mashimad@hiroshima-u.ac.jp. | lld:pubmed |
| pubmed-article:15231699 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15231699 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15231699 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:497061 | entrezgene:pubmed | pubmed-article:15231699 | lld:entrezgene |
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