Linked Life Data

15212789

Source:http://linkedlifedata.com/resource/pubmed/id/15212789

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pubmed-article:15212789pubmed:abstractTextThe cyanobacterial protein NtcA is a global transcriptional regulator of genes involved in nitrogen assimilation that are subjected to ammonium-promoted repression and is itself controlled by positive autoregulation. Strain CSI70 derived from Synechococcus sp. strain PCC 7942 was constructed to overexpress an additional ntcA gene copy from a constitutive promoter. This strain exhibited high levels of the NtcA protein both in the presence and in the absence of ammonium. However, expression of the NtcA-dependent nir operon and glnA gene (tested by RNA/DNA hybridization and enzyme activity) was still subjected to nitrogen regulation. These results indicate in vivo regulation of the activity of NtcA at activation of transcription of nitrogen-regulated genes as a function of the nitrogen status of the cell.lld:pubmed
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pubmed-article:15212789pubmed:dateRevised2008-11-21lld:pubmed
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pubmed-article:15212789pubmed:articleTitleIn vivo activity of the nitrogen control transcription factor NtcA is subjected to metabolic regulation in Synechococcus sp. strain PCC 7942.lld:pubmed
pubmed-article:15212789pubmed:affiliationInstituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Avda. Americo Vespucio s/n, E-41092 Seville, Spain.lld:pubmed
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pubmed-article:15212789pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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