Linked Life Data

15201169

Source:http://linkedlifedata.com/resource/pubmed/id/15201169

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-6-17
pubmed:abstractText
There are currently no effective treatments to restore the cardiac muscle lost because of ischemia for the millions of people who suffer heart attacks annually. Cell therapy procedures have emerged as novel therapeutic strategies for treatment of heart failure after myocardial infarction but have been hampered by the lack of adequate cell sources of cardiomyocytes and by the inability to integrate cell grafts into cardiac muscle. A cardiac patch composed of organized and functional cardiomyocytes could drastically enhance the efficacy of this important clinical approach. Here, we report our ongoing efforts to develop a bioartificial cardiac muscle capable of synchronized multidirectional contraction within a three-dimensional hydrogel scaffold. Neonatal rat cardiomyocytes, smooth muscle cells, and reconstituted polymeric collagen enriched with growth factors and hormones are used. A bioreactor system is used to impart precise strains onto the developing tissue constructs in vitro. The results demonstrate that cell-mediated collagen compaction is significantly enhanced by strain preconditioning, resulting in a more favorable cellular organization. Furthermore, the results demonstrate that strain stimulation guides cellular orientation in the direction of applied strain (i.e., in the circumferential direction). Hence, we demonstrate the importance of mechanical preconditioning as a means of promoting the in vitro development of engineered cardiac muscle for use with myocardial regeneration therapies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0077-8923
pubmed:author
pubmed:issnType
Print
pubmed:volume
1015
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
299-311
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Controlling the cellular organization of tissue-engineered cardiac constructs.
pubmed:affiliation
Department of Biomedical Engineering, Technion IIT, Haifa, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't