Source:http://linkedlifedata.com/resource/pubmed/id/15104122
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
16
|
| pubmed:dateCreated |
2004-4-23
|
| pubmed:abstractText |
Biochemical and genetic studies have demonstrated that transcription factors encoded by the E2A gene are essential in regulating B lineage specific gene expression and B lineage commitment. However, the mechanism by which E2A regulates B lineage commitment is not known. It has been reported that E2A controls B lineage commitment in a dosage dependent manner. To further investigate this gene dosage effect, we analyzed E2A expression during normal B cell development in mice carrying a functional E2AGFP knockin allele. Mice carrying this fusion allele were examined for E2A gene expression during bone marrow B cell development. A dramatic upregulation of E2A is observed concomitant with the initiation of immunoglobulin heavy chain D-J rearrangement and the induction of Early B cell Factor (EBF) gene expression. We also show that this E2A upregulation does not occur in the absence of the EBF gene. These results indicate that E2A upregulation is a critical step in regulating B-lineage commitment. It further suggests that E2A gene dosage may be determined by a cross regulation between E2A and EBF during B lineage commitment.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0161-5890
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
40
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1165-77
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:15104122-Alleles,
pubmed-meshheading:15104122-Animals,
pubmed-meshheading:15104122-B-Lymphocytes,
pubmed-meshheading:15104122-Bone Marrow Cells,
pubmed-meshheading:15104122-Cell Differentiation,
pubmed-meshheading:15104122-Cell Line,
pubmed-meshheading:15104122-Cell Lineage,
pubmed-meshheading:15104122-Cell Transformation, Viral,
pubmed-meshheading:15104122-Gene Dosage,
pubmed-meshheading:15104122-Gene Expression Regulation,
pubmed-meshheading:15104122-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:15104122-Gene Targeting,
pubmed-meshheading:15104122-Genes, Immunoglobulin,
pubmed-meshheading:15104122-Hematopoietic Stem Cells,
pubmed-meshheading:15104122-Homozygote,
pubmed-meshheading:15104122-Lymphopoiesis,
pubmed-meshheading:15104122-Mice,
pubmed-meshheading:15104122-Mice, Inbred C57BL,
pubmed-meshheading:15104122-Mice, Inbred Strains,
pubmed-meshheading:15104122-Transcription Factors
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Regulation of E2A gene expression in B-lymphocyte development.
|
| pubmed:affiliation |
Department of Immunology, Duke University Medical Center, Jones Building Room 329, Box 3010, Durham, NC 27710, USA. yzhuang@duke.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|