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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2004-4-22
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pubmed:abstractText |
We tested the hypothesis that cathepsins and specifically toxopain-1, a cathepsin B, play a critical role in the pathogenesis of toxoplasmosis. We found that inhibiting the expression of toxopain-1-specific mRNA and protein by >60% significantly decreased the capacity of the parasites to multiply and invade in vitro. To relate these in vitro results to the role of toxopain-1 in pathogenesis in vivo, we developed a novel chicken embryo model of congenital toxoplasmosis. Inhibiting either toxopain-1 expression or specific cysteine proteinase activity significantly reduced congenital infection of chicken embryos, as determined by histopathology and by the number of parasites quantified by real-time PCR. Our new model provides key in vivo validation for the hypothesis that toxopain-1 is a potential drug target in Toxoplasma gondii and also provides a new animal model for rapid, inexpensive screening of antiparasitic compounds.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-10542284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-10792730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-10943525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-10989144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-11716777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-11849701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-12000756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-12471262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-12711703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-1520757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-3183382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-7707373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-8054436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-8459830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-9371086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-9566522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-9705954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-9708737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15102804-9988756
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/toxopain-1, Toxoplasma gondii
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2915-21
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15102804-Animals,
pubmed-meshheading:15102804-Base Sequence,
pubmed-meshheading:15102804-Chick Embryo,
pubmed-meshheading:15102804-Cysteine Endopeptidases,
pubmed-meshheading:15102804-DNA, Protozoan,
pubmed-meshheading:15102804-Disease Models, Animal,
pubmed-meshheading:15102804-Gene Expression,
pubmed-meshheading:15102804-Genes, Protozoan,
pubmed-meshheading:15102804-Humans,
pubmed-meshheading:15102804-RNA, Antisense,
pubmed-meshheading:15102804-RNA, Messenger,
pubmed-meshheading:15102804-RNA, Protozoan,
pubmed-meshheading:15102804-Species Specificity,
pubmed-meshheading:15102804-Toxoplasma,
pubmed-meshheading:15102804-Toxoplasmosis, Animal,
pubmed-meshheading:15102804-Toxoplasmosis, Cerebral,
pubmed-meshheading:15102804-Toxoplasmosis, Congenital
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pubmed:year |
2004
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pubmed:articleTitle |
Toxopain-1 is critical for infection in a novel chicken embryo model of congenital toxoplasmosis.
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pubmed:affiliation |
Departments of Pathology and Medicine, University of California, San Diego, California 92103-8416, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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