Linked Life Data

15100788

Source:http://linkedlifedata.com/resource/pubmed/id/15100788

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-4-21
pubmed:abstractText
We report a protocol for on-chip electrophoretic sample loading and sample component separation in which each operation requires simultaneous control of the potential of only two electrodes: during the sample-loading phase, the potentials at the ends of the separation channel are electrically floating; during electrophoresis of the sample mixture down the separation channel, the potentials at the ends of the sample-introduction channel are floating. This method, which we call "floating-stacking," avoids the dispersion/distortion of the sample plug that is commonly associated with simultaneous electrical control of only two electrodes in a crossed-channel or offset-double-tee injection system. Further, when this floating loading/separation is done in the presence of back-transient-isotachophoresis, sample loss from the plug of material being injected is minimal and a significant concentration increase--up to 13x--of the sample components in the separated bands occurs relative to the commonly used "pinch-and-pull-back" technique (which requires simultaneous electrical control of four electrodes).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1473-0197
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
86-92
pubmed:dateRevised
2011-11-2
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Use of floating electrodes in transient isotachophoresis to increase the sensitivity of detection.
pubmed:affiliation
ACLARA BioSciences, Inc, 1288 Pear Avenue, Mountain View, CA 94043, USA.
pubmed:publicationType
Journal Article, Comparative Study