Linked Life Data

15082186

Source:http://linkedlifedata.com/resource/pubmed/id/15082186

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2004-4-14
pubmed:abstractText
Whole-cell voltage-clamp recordings from dissociated hair cells of the statocyst of octopus, Eledone cirrhosa, demonstrated that application of ACh, carbachol or muscarine (10 microM) reversibly decreased the amplitude of L-type calcium current (I(Ca,L)), while nicotine (10-100 microM) did not have any effect. Furthermore, atropine blocked the effect of ACh and agonists suggesting that ACh reduces I(Ca,L) through activation of muscarinic receptors. Internal dialysis of these cells with guanosine 5'-O-3-thiotriphosphate (GTPgammaS), a non-hydrolysable GTP analogue, mimicked the ACh-induced inhibition of I(Ca,L) and occluded any further ACh-induced inhibition. Internal dialysis of these hair cells with guanosine 5'-O-(2-thiodiphosphate) (GDPbetaS) reduced the ACh-induced inhibition I(Ca,L). The inhibitory effects of ACh were abolished by pre-incubation of these cells with pretussis toxin (PTX) suggesting that ACh-induced inhibition of I(Ca,L) involves a PTX-sensitive G protein pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
360
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
90-4
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Cholinergic modulation of L-type calcium current in isolated sensory hair cells of the statocyst of octopus, Eledone cirrhosa.
pubmed:affiliation
University of Plymouth, School of Biological Sciences, Drake Circus, Plymouth PL4 8AA, UK. aacc@mba.ac.uk
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't