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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2004-3-16
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pubmed:abstractText |
The fidelity of chromosome segregation requires that the cohesin protein complex bind together newly replicated sister chromatids both at centromeres and at discrete sites along chromosome arms. Segregation of the yeast 2 micro plasmid also requires cohesin, which is recruited to the plasmid partitioning locus. Here we report that the RSC chromatin-remodeling complex regulates the differential association of cohesin with centromeres and chromosome arms. RSC cycles on and off chromosomal arm and plasmid cohesin binding sites in a cell cycle-regulated manner 15 min preceding Mcd1p, the central cohesin subunit. We show that in rsc mutants Mcd1p fails to associate with chromosome arms but still binds to centromeres, and that consequently, the arm regions of mitotic sister chromosomes separate precociously while cohesion at centromeres is unaffected. Our data suggest a role for RSC in facilitating the loading of cohesin specifically onto chromosome arms, thereby ensuring sister chromatid cohesion and proper chromosome segregation.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MCD1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RSC complex, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/cohesins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
739-50
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15023343-Binding Sites,
pubmed-meshheading:15023343-Cell Cycle Proteins,
pubmed-meshheading:15023343-Cell Division,
pubmed-meshheading:15023343-Centromere,
pubmed-meshheading:15023343-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:15023343-Chromosome Segregation,
pubmed-meshheading:15023343-Chromosomes, Fungal,
pubmed-meshheading:15023343-DNA-Binding Proteins,
pubmed-meshheading:15023343-Fungal Proteins,
pubmed-meshheading:15023343-Mutation,
pubmed-meshheading:15023343-Nuclear Proteins,
pubmed-meshheading:15023343-Phosphoproteins,
pubmed-meshheading:15023343-Plasmids,
pubmed-meshheading:15023343-Protein Structure, Tertiary,
pubmed-meshheading:15023343-Saccharomyces cerevisiae,
pubmed-meshheading:15023343-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:15023343-Transcription Factors
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pubmed:year |
2004
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pubmed:articleTitle |
The RSC nucleosome-remodeling complex is required for Cohesin's association with chromosome arms.
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pubmed:affiliation |
Program in Molecular and Cellular Biology, Department of Microbiology and Immunology, Morse Institute of Molecular Biology and Genetics, SUNY Downstate Medical Center, Brooklyn, NY 11203, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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