Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-3-15
pubmed:abstractText
The major pathway of mRNA decay in yeast initiates with deadenylation, followed by mRNA decapping and 5'-3' exonuclease digestion. An in silico approach was used to identify new proteins involved in the mRNA decay pathway. One such protein, Edc3p, was identified as a conserved protein of unknown function having extensive two-hybrid interactions with several proteins involved in mRNA decapping and 5'-3' degradation including Dcp1p, Dcp2p, Dhh1p, Lsm1p, and the 5'-3' exonuclease, Xrn1p. We show that Edc3p can stimulate mRNA decapping of both unstable and stable mRNAs in yeast when the decapping enzyme is compromised by temperature-sensitive alleles of either the DCP1 or the DCP2 genes. In these cases, deletion of EDC3 caused a synergistic mRNA-decapping defect at the permissive temperatures. The edc3Delta had no effect when combined with the lsm1Delta, dhh1Delta, or pat1Delta mutations, which appear to affect an early step in the decapping pathway. This suggests that Edc3p specifically affects the function of the decapping enzyme per se. Consistent with a functional role in decapping, GFP-tagged Edc3p localizes to cytoplasmic foci involved in mRNA decapping referred to as P-bodies. These results identify Edc3p as a new protein involved in the decapping reaction.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-10101156, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-10471698, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-10688190, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-10690409, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-10747033, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-10761922, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-10900456, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-10913177, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11027264, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11027292, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11101803, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11239395, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11284008, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11410650, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11514438, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11691829, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11741542, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11780629, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11805826, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-11889048, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12032091, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12073375, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12140549, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12417715, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12486012, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12515382, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12554866, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12554867, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-12730603, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-1427074, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-7926773, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-8346213, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-8355674, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-8757137, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-8816497, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-9207794, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-9482746, http://linkedlifedata.com/resource/pubmed/commentcorrection/15020463-9717241
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
166
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
729-39
pubmed:dateRevised
2011-1-5
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Identification of Edc3p as an enhancer of mRNA decapping in Saccharomyces cerevisiae.
pubmed:affiliation
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson 85721-0106, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't