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pubmed-article:1468572pubmed:abstractTextThe unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.lld:pubmed
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pubmed-article:1468572pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1468572pubmed:articleTitlePresence and coding properties of 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um) in the wobble position of the anticodon of tRNA(Leu) (U*AA) from brewer's yeast.lld:pubmed
pubmed-article:1468572pubmed:affiliationLaboratoire de Biochemie Médicale, Faculté de Médecine, Université de Bourgogne, Dijon, France.lld:pubmed
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pubmed-article:1468572pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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