Linked Life Data

14676831

Source:http://linkedlifedata.com/resource/pubmed/id/14676831

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2004-4-1
pubmed:abstractText
Microtubules play a central role in coordinating various cellular functions that are orchestrated by their interaction with molecular motors. Anticancer drugs that target microtubule dynamics have been shown to be effective in cancer treatment. However, the effect of microtubule motor-associated molecules on cancer cell proliferation is not clear. Here, we investigated the role of NudC, a nuclear movement protein associated with the microtubule motor dynein, on prostate tumorigenesis. Recombinant adenovirus expressing NudC (Ad-NudC) was used to examine the effects of NudC on the tumorigenicity of prostate cancer cells. Expression of NudC in LNCaP cells inhibited their anchorage-independent growth in a soft agar colony assay. Expression of NudC in DU145 or PC-3 cells inhibited tumor growth in a subcutaneous xenograft model. At the cellular level, expression of NudC in DU145 and PC-3 cells inhibited cell proliferation at 48 h after Ad-NudC infection. FACS analysis of cell cycle distribution showed that 50-60% of Ad-NudC-infected PC-3 cells have a G2/M-phase DNA content compared to about 16-19% in Ad-Luciferase (Ad-Luc)-infected control cells, suggesting that NudC overexpression resulted in aberrant cell cycle progression. Immunofluorescence microscopy revealed a significant increase in cells with a single enlarged nucleus and cells exhibiting multiple nuclei, along with a concomitant increase in cell size in Ad-NudC-infected cells. These results suggest that NudC overexpression led to a block in cell division of prostate cancer cells, and that Ad-NudC may provide a new anticancer drug approach targeting the function of a microtubule motor-associated protein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2499-506
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:14676831-Adenoviridae, pubmed-meshheading:14676831-Animals, pubmed-meshheading:14676831-Cell Adhesion, pubmed-meshheading:14676831-Cell Cycle Proteins, pubmed-meshheading:14676831-Cell Division, pubmed-meshheading:14676831-Cell Line, Tumor, pubmed-meshheading:14676831-Cell Size, pubmed-meshheading:14676831-DNA, pubmed-meshheading:14676831-Dyneins, pubmed-meshheading:14676831-G2 Phase, pubmed-meshheading:14676831-Gene Expression, pubmed-meshheading:14676831-Gene Transfer Techniques, pubmed-meshheading:14676831-Humans, pubmed-meshheading:14676831-Male, pubmed-meshheading:14676831-Mice, pubmed-meshheading:14676831-Mice, Nude, pubmed-meshheading:14676831-Microtubule-Associated Proteins, pubmed-meshheading:14676831-Models, Biological, pubmed-meshheading:14676831-Neoplasm Transplantation, pubmed-meshheading:14676831-Nuclear Proteins, pubmed-meshheading:14676831-Prostatic Neoplasms, pubmed-meshheading:14676831-Proteins, pubmed-meshheading:14676831-Recombinant Proteins, pubmed-meshheading:14676831-Transplantation, Heterologous
pubmed:year
2004
pubmed:articleTitle
Inhibition of prostate tumor growth by overexpression of NudC, a microtubule motor-associated protein.
pubmed:affiliation
Department of Molecular Pathology, MD Anderson Cancer Center, Houston, TX 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't