@prefix activity: .
@prefix address: .
@prefix agency: .
@prefix assay: .
@prefix assayCategory: .
@prefix assayTargetLink: .
@prefix biopax3: .
@prefix calbc: .
@prefix calbc-group: .
@prefix carrierLink: .
@prefix certainAgreement: .
@prefix chebi: .
@prefix chembl: .
@prefix chemicalCompound: .
@prefix chemicalCompoundRecord: .
@prefix clan: .
@prefix clinicalResult: .
@prefix clinicalStudy: .
@prefix clinicaltrials: .
@prefix company: .
@prefix confidenceScore: .
@prefix contact: .
@prefix country: .
@prefix cpath: .
@prefix curationLookup: .
@prefix dailymed: .
@prefix dailymed-drugs: .
@prefix dailymed-ingredient: .
@prefix dailymed-instance: .
@prefix databaseReference: .
@prefix dc: .
@prefix dc-term: .
@prefix diseaseontology: .
@prefix diseasome: .
@prefix diseasome-diseases: .
@prefix diseasome-gene: .
@prefix diseasome-instance: .
@prefix documentType: .
@prefix dosageForm: .
@prefix drug: .
@prefix drugbank: .
@prefix drugbank-proteinSequence: .
@prefix drugCategory: .
@prefix drugInteraction: .
@prefix drugTarget: .
@prefix eligibility: .
@prefix entrez-gene: .
@prefix entrez-goterm: .
@prefix entrezgene: .
@prefix enzymeLink: .
@prefix externalIdentifier: .
@prefix externalLink: .
@prefix factforge: .
@prefix family: .
@prefix foaf: .
@prefix formulation: .
@prefix freebase: .
@prefix gene-rif: .
@prefix geneontology: .
@prefix geneSequence: .
@prefix hapmap: .
@prefix indication: .
@prefix intervention: .
@prefix investigator: .
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@prefix lifeskim: .
@prefix linkedct: .
@prefix linkedct-condition: .
@prefix linkedct-intervention: .
@prefix literatureCitation: .
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@prefix measurement: .
@prefix meddraSideEffect: .
@prefix mixture: .
@prefix molecularSpecies: .
@prefix molecule: .
@prefix moleculeType: .
@prefix ontotext: .
@prefix organismSpecies: .
@prefix outcomeAnalysis: .
@prefix outcomeMeasure: .
@prefix overallOfficial: .
@prefix owl: .
@prefix owlim: .
@prefix packager: .
@prefix participantFlow: .
@prefix patent: .
@prefix pfam: .
@prefix phenotype: .
@prefix pointOfContact: .
@prefix price: .
@prefix primaryOutcome: .
@prefix product: .
@prefix property: .
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@prefix pubmed: .
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@prefix result: .
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@prefix secondaryOutcome: .
@prefix sideEffect: .
@prefix sideEffectFrequency: .
@prefix sider: .
@prefix sider-drug: .
@prefix skos: .
@prefix skos-xl: .
@prefix snp: .
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@prefix symptom: .
@prefix synonym: .
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@prefix transporterLink: .
@prefix umls: .
@prefix umls-concept: .
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@prefix umls-semnetwork: .
@prefix uniprot: .
@prefix uniprot-protein: .
@prefix uniprot-unstable: .
@prefix xsd: .
pubmed-article:14645242 a pubmed:Citation ;
lifeskim:mentions umls-concept:C0031715 , umls-concept:C0528561 ;
pubmed:issue "7" ;
pubmed:dateCreated "2004-2-9" ;
pubmed:abstractText "Akt negatively regulates apoptotic pathways at a premitochondrial level through phosphorylation and modulation of proteins such as Bad, Forkhead proteins, and GSK-3beta. Akt has also been shown to protect cell death at a post-mitochondrial level, although its downstream targets have not been well documented. Here, we demonstrate that Akt, including AKT1 and AKT2, interacts with and phosphorylates X-linked inhibitor of apoptosis protein (XIAP) at residue serine-87 in vitro and in vivo. Phosphorylation of XIAP by Akt protects XIAP from ubiquitination and degradation in response to cisplatin. Moreover, autoubiquitination of XIAP is also inhibited by Akt. Consistent with this, an XIAP mutant introduced into cells which mimics the Akt-phosphorylated form (i.e. XIAP-S87D) displays reduced ubiquitination and degradation as compared with wild type XIAP. The greater stability of XIAP-S87D in cells translated to increased cell survival after cisplatin treatment. Conversely, a mutant that could not be phosphorylated by Akt (XIAP-S87A) was more rapidly degraded and showed increased cisplatin-induced apoptosis. Furthermore, suppression of XIAP by either siRNA or adenovirus of antisense of XIAP induced programmed cell death and inhibited Akt-stimulated cell survival in ovarian cancer cells. These data identify XIAP as a new downstream target of Akt and a potentially important mediator of the effect of Akt on cell survival." ;
pubmed:grant , ;
pubmed:language "eng" ;
pubmed:journal ;
pubmed:citationSubset "IM" ;
pubmed:chemical , , , , , , , , , , , , , , , , , , , , , , , , , ;
pubmed:status "MEDLINE" ;
pubmed:month "Feb" ;
pubmed:issn "0021-9258" ;
pubmed:author , , pubmed-author:WangHong-GangHG , pubmed-author:SunMeiM , pubmed-author:KanekoSatoshiS , , , ;
pubmed:issnType "Print" ;
pubmed:day "13" ;
pubmed:volume "279" ;
pubmed:owner "NLM" ;
pubmed:authorsComplete "Y" ;
pubmed:pagination "5405-12" ;
pubmed:dateRevised "2011-11-17" ;
pubmed:meshHeading pubmed-meshheading:14645242-Humans , pubmed-meshheading:14645242-Animals , pubmed-meshheading:14645242-Mice , pubmed-meshheading:14645242-Proteins , pubmed-meshheading:14645242-Serine , pubmed-meshheading:14645242-Rats , pubmed-meshheading:14645242-Mutation , pubmed-meshheading:14645242-Phosphorylation , , , , , , , , pubmed-meshheading:14645242-Plasmids , , pubmed-meshheading:14645242-Cisplatin , , pubmed-meshheading:14645242-Apoptosis , , , , pubmed-meshheading:14645242-Immunoblotting , , , , pubmed-meshheading:14645242-Ubiquitin , , , , pubmed-meshheading:14645242-Caspases , , , , ;
pubmed:year "2004" ;
pubmed:articleTitle "Akt phosphorylation and stabilization of X-linked inhibitor of apoptosis protein (XIAP)." ;
pubmed:affiliation "Departments of Pathology and Interdisciplinary Oncology, University of South Florida College of Medicine and H. Lee Moffitt Cancer Center, Tampa, Florida 33612, USA." ;
pubmed:publicationType "Journal Article" , "Research Support, U.S. Gov't, P.H.S." , "Research Support, U.S. Gov't, Non-P.H.S." , "Research Support, Non-U.S. Gov't" .