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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-12-3
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pubmed:abstractText |
EN-RAGE is a ligand for the receptor for advanced glycation end products (RAGE) and may be involved in the development of diabetic macro- and micro-angiopathy. This study is designed to investigate the regulation of EN-RAGE gene expression in human macrophages. The amounts of EN-RAGE mRNA were measured in cultured human THP-1 macrophages after treatment with various stimuli known to modulate atherosclerosis. First, interleukin-6 (IL-6), a proinflammatory cytokine, increased the level of EN-RAGE mRNA by approximately 2-fold in a time- and a dose-dependent fashion. EN-RAGE protein was detected in the cultured medium and increased significantly by the addition of IL-6. The induction was abolished by pretreatment with the JAK kinase inhibitor and cycloheximide, but not with the MEK kinase inhibitor. Second, pioglitazone (PIO), a thiazolidinedione, decreased the level of EN-RAGE mRNA by approximately 25% of the basal in a time- and a dose-dependent fashion. Pioglitazone also inhibited the induction of EN-RAGE mRNA by IL-6. These results indicate the production of EN-RAGE is induced by IL-6 through de novo protein synthesis via the JAK-STAT kinase pathway and inhibited by the activation of peroxisome proliferator-activated receptor-gamma (PPARgamma) in human macrophages.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/S100A12 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazolidinediones,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/pioglitazone
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9150
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pubmed:author |
pubmed-author:HasegawaTakamasaT,
pubmed-author:Inoue-ShibataMegumiM,
pubmed-author:IwasakaToshijiT,
pubmed-author:KimuraTatsujiT,
pubmed-author:KimuraYutakaY,
pubmed-author:KosakiAtsushiA,
pubmed-author:MasakiHiroyaH,
pubmed-author:MatsubaraHiroakiH,
pubmed-author:MoriYasukiyoY,
pubmed-author:NishikawaMitsushigeM,
pubmed-author:OkigakiMitsuhikoM,
pubmed-author:ToyodaNagaokiN
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pubmed:copyrightInfo |
Copyright 2003 Elsevier Ireland Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
171
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
211-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14644389-Analysis of Variance,
pubmed-meshheading:14644389-Base Sequence,
pubmed-meshheading:14644389-Cell Line, Tumor,
pubmed-meshheading:14644389-Down-Regulation,
pubmed-meshheading:14644389-Enzyme Activation,
pubmed-meshheading:14644389-Gene Expression Regulation,
pubmed-meshheading:14644389-Humans,
pubmed-meshheading:14644389-Interleukin-6,
pubmed-meshheading:14644389-MAP Kinase Signaling System,
pubmed-meshheading:14644389-Macrophages,
pubmed-meshheading:14644389-RNA, Messenger,
pubmed-meshheading:14644389-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:14644389-S100 Proteins,
pubmed-meshheading:14644389-Signal Transduction,
pubmed-meshheading:14644389-Thiazolidinediones,
pubmed-meshheading:14644389-Transcription Factors,
pubmed-meshheading:14644389-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
The regulation of EN-RAGE (S100A12) gene expression in human THP-1 macrophages.
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pubmed:affiliation |
Department of Medicine II, Kansai Medical University, 10-15 Fumizono-cho, Osaka 570-8507, Moriguchi, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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