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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2003-11-4
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pubmed:abstractText |
The type V TGF-beta receptor (TbetaR-V)/IGFBP-3 receptor mediates the IGF-independent growth inhibition induced by IGFBP-3. It also mediates the growth inhibitory response to TGF-beta1 in concert with other TGF-beta receptor types, and its loss may contribute to the malignant phenotype of human carcinoma cells. Here we demonstrate that TbetaR-V is identical to LRP-1/alpha2M receptor as shown by MALDI-TOF analysis of tryptic peptides of TbetaR-V purified from bovine liver. In addition, 125I-IGFBP-3 affinity-labeled TbetaR-V in Mv1Lu cells is immunoprecipitated by antibodies to LRP-1 and TbetaR-V. RAP, an LRP-1 antagonist, inhibits binding of 125I-TGF-beta1 and 125I-IGFBP-3 to TbetaR-V and diminishes IGFBP-3-induced growth inhibition in Mv1Lu cells. Absent or low levels of LRP-1, as with TbetaR-V, have been linked to the malignant phenotype of carcinoma cells. Mutagenized Mv1Lu cells selected for reduced expression of LRP-1 have an attenuated growth inhibitory response to TGF-beta1 and IGFBP-3. LRP-1-deficient mouse embryonic fibroblasts lack a growth inhibitory response to TGF-beta1 and IGFBP-3. On the other hand, stable transfection of H1299 human lung carcinoma cells with LRP-1 cDNA restores the growth inhibitory response. These results suggest that the LRP-1/TbetaR-V/IGFBP-3 receptor is required for the growth inhibitory response to IGFBP-3 and TGF-beta1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Low Density Lipoprotein...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transforming Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1530-6860
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2068-81
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14597676-Animals,
pubmed-meshheading:14597676-Carcinoma,
pubmed-meshheading:14597676-Cattle,
pubmed-meshheading:14597676-Cell Division,
pubmed-meshheading:14597676-Cell Line, Tumor,
pubmed-meshheading:14597676-Cells, Cultured,
pubmed-meshheading:14597676-Humans,
pubmed-meshheading:14597676-Insulin-Like Growth Factor Binding Protein 3,
pubmed-meshheading:14597676-Liver,
pubmed-meshheading:14597676-Low Density Lipoprotein Receptor-Related Protein-1,
pubmed-meshheading:14597676-Lung Neoplasms,
pubmed-meshheading:14597676-Mice,
pubmed-meshheading:14597676-Mice, Knockout,
pubmed-meshheading:14597676-Precipitin Tests,
pubmed-meshheading:14597676-Receptors, Cell Surface,
pubmed-meshheading:14597676-Receptors, Transforming Growth Factor beta,
pubmed-meshheading:14597676-Sequence Homology, Amino Acid,
pubmed-meshheading:14597676-Transfection,
pubmed-meshheading:14597676-Transforming Growth Factor beta,
pubmed-meshheading:14597676-Transforming Growth Factor beta1
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pubmed:year |
2003
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pubmed:articleTitle |
Cellular growth inhibition by IGFBP-3 and TGF-beta1 requires LRP-1.
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pubmed:affiliation |
Department of Biochemistry, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104, USA. huangss@slu.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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