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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-10-23
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pubmed:abstractText |
GCM proteins constitute a small transcription factor family. Nuclear localization of Drosophila GCM is mediated by a typical bipartite nuclear localization sequence (NLS) close to the DNA-binding GCM domain. Here, we have analyzed nuclear localization of the mammalian GCM proteins. Whereas GCMb/Gcm-2 contained a classical bipartite NLS, nuclear localization of GCMa/Gcm-1 was mediated by two regions without resemblance to known NLS, one corresponding to the amino-terminal part of the GCM domain, the second defined as a tyrosine-and-proline-rich carboxy-terminal region. Nuclear import was counteracted by an amino-terminal nuclear export activity. This complex regulation of subcellular localization has important implications for GCMa/Gcm-1 function.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
553
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
315-20
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14572643-Active Transport, Cell Nucleus,
pubmed-meshheading:14572643-Amino Acid Sequence,
pubmed-meshheading:14572643-Animals,
pubmed-meshheading:14572643-Base Sequence,
pubmed-meshheading:14572643-Cell Nucleus,
pubmed-meshheading:14572643-DNA-Binding Proteins,
pubmed-meshheading:14572643-Galactosidases,
pubmed-meshheading:14572643-Genetic Complementation Test,
pubmed-meshheading:14572643-HeLa Cells,
pubmed-meshheading:14572643-Humans,
pubmed-meshheading:14572643-Mice,
pubmed-meshheading:14572643-Molecular Sequence Data,
pubmed-meshheading:14572643-NIH 3T3 Cells,
pubmed-meshheading:14572643-Neuropeptides,
pubmed-meshheading:14572643-Nuclear Localization Signals,
pubmed-meshheading:14572643-Nuclear Proteins,
pubmed-meshheading:14572643-Protein Structure, Tertiary,
pubmed-meshheading:14572643-Recombinant Fusion Proteins,
pubmed-meshheading:14572643-Trans-Activators,
pubmed-meshheading:14572643-Transfection
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pubmed:year |
2003
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pubmed:articleTitle |
Structural requirements for nuclear localization of GCMa/Gcm-1.
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pubmed:affiliation |
Institut für Biochemie, Universität Erlangen-Nürnberg, Fahrstrasse 17, D-91054 Erlangen, Germany. sh@biochem.uni-erlangen.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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