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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-10-21
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pubmed:abstractText |
During the first trimester of pregnancy, the human placenta is an actively dividing and highly invasive tumour-like tissue, while near term, it represents a fully developed, non-invasive unit. In order to understand the molecular basis of this marked difference in the placental phenotypes, an approach based on a differential display-reverse transcription-polymerase chain reaction (DD-RT-PCR) was adopted to analyse changes in gene expression, using total RNA isolated from first-trimester and term placental villi. Using this approach, T-plastin was initially identified as being differentially expressed in the human first-trimester placenta. T-plastin is an actin-bundling protein and is known to be highly expressed in actively dividing cells and up-regulated in several carcinomas. Using a homogenous population of cytotrophoblasts and syncytiotrophoblasts isolated from human placentae, the present authors demonstrate the differential expression of T-plastin in cytotrophoblasts compared with the terminally differentiated syncytiotrophoblasts. The down-regulation of T-plastin expression is further demonstrated in human trophoblastic BeWo cells induced to differentiate using transforming growth factor (TGF)beta1, a growth factor known for its anti-proliferative and anti-invasive response in placental cells. These studies suggest that expression of T-plastin in the placental context may indeed be associated with the enhanced replicative potential of placental trophoblasts.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PAEP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pregnancy Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1,
http://linkedlifedata.com/resource/pubmed/chemical/plastin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1472-6483
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
235-42
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:14567899-Blotting, Western,
pubmed-meshheading:14567899-Cell Differentiation,
pubmed-meshheading:14567899-Cell Division,
pubmed-meshheading:14567899-DNA, Complementary,
pubmed-meshheading:14567899-Endometrium,
pubmed-meshheading:14567899-Female,
pubmed-meshheading:14567899-Gene Expression Regulation, Developmental,
pubmed-meshheading:14567899-Glycoproteins,
pubmed-meshheading:14567899-Humans,
pubmed-meshheading:14567899-Membrane Glycoproteins,
pubmed-meshheading:14567899-Microfilament Proteins,
pubmed-meshheading:14567899-Phosphoproteins,
pubmed-meshheading:14567899-Pregnancy,
pubmed-meshheading:14567899-Pregnancy Proteins,
pubmed-meshheading:14567899-Pregnancy Trimester, First,
pubmed-meshheading:14567899-RNA, Messenger,
pubmed-meshheading:14567899-Transforming Growth Factor beta,
pubmed-meshheading:14567899-Transforming Growth Factor beta1,
pubmed-meshheading:14567899-Trophoblasts
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pubmed:year |
2003
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pubmed:articleTitle |
Changes in T-plastin expression with human trophoblast differentiation.
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pubmed:affiliation |
Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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