Linked Life Data

1383548

Source:http://linkedlifedata.com/resource/pubmed/id/1383548

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-11-6
pubmed:abstractText
Outwardly rectifying Cl- channels in cultured human Jurkat T-lymphocytes were activated by excising a patch of membrane using the inside-out (i/o) patch-clamp configuration and holding at depolarized voltages for prolonged periods of time (1-6 min at +80 mV, 20 degrees C). The single-channel current at +80 mV was 4.5 +/- 0.3 pA and at -80 mV, it was 1.0 +/- 0.4 pA. After activation, the probability of being open (Po) for the lymphocyte channel was voltage independent. Activation of the Cl- channel in lymphocytes was temperature dependent. Nineteen percent of i/o recordings from lymphocytes made at 20 degrees C exhibited Cl- channel activity. In contrast, 49% of recordings made at 30 degrees C showed channel activity. The number of channels in an active patch was not significantly different at the two temperatures. Channel activation in excised, depolarized patches also occurred 20-fold faster at 30 degrees C than at 20 degrees C. There was no marked change in the single-channel conductance at 30 degrees C. Open-channel conductance was blocked by 200 microM indanyloxyacetic acid (IAA) or 1 mM SITS when applied to the intracellular side of the patch. The characteristics of this channel are similar to epithelial outwardly rectifying Cl- channels thought to be involved in fluid secretion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:volume
127
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-56
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Outwardly rectifying chloride channels in lymphocytes.
pubmed:affiliation
Department of Neurobiology, Stanford University, California 94305-5401.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't