Linked Life Data

1377510

Source:http://linkedlifedata.com/resource/pubmed/id/1377510

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-7-31
pubmed:abstractText
We have studied Ca2+ voltage-gated channels of immature rat Sertoli cells by measuring intracellular Ca2+ concentration and its variation following administration of various agents in fura-2-loaded, confluent monolayers in culture. Our findings indicate that the basal Ca2+ intracellular level is about 100 nM, a value that falls within the range found in most eukaryotic cells. The intracellular Ca2+ level is rapidly increased by fetal bovine serum through release of intracellularly stored Ca2+ and opening of membrane cation channels. Substantial Ca2+ influx in rat Sertoli cells seems to be mediated by voltage-gated cation channels, which are sensitive to nifedipine, nicardipine, and omega-conotoxin. To investigate whether FSH, which controls several morphological and biochemical events of prepubertal Sertoli cells, modified Ca2+ influx in this cell type, we analyzed the cell response to acute FSH administration. The results show that, although not influencing the basal concentration of Ca2+, FSH decreases intracellular calcium influx induced by membrane depolarization. Similar data were also obtained by adding dibutyryl cAMP to the external medium and by increasing endogenous cAMP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
414-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Voltage-gated calcium channels in rat Sertoli cells.
pubmed:affiliation
Institute of Histology and General Embryology, University La Sapienza, Rome, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't