Linked Life Data

1368661

Source:http://linkedlifedata.com/resource/pubmed/id/1368661

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-7-11
pubmed:abstractText
Until recently, it was assumed that any short DNA segment could be regarded as a straight rod. Many instances, however, have been reported in which the helical axis was curved. In this study, to gain a general insight into the structural features of sequence-directed DNA curvatures, we constructed a set of plasmids carrying curved DNA segments, which were randomly cloned from Streptomyces total DNA with extremely high G/C-contents. The results of primary characterization of these curved DNA segments are presented. Although the cloned DNA segments had high G/C-contents, in all cases, several homopolymeric [dA] and [dT] stretches were found to be clustered around the centres of the determined nucleotide sequences. Furthermore, the clustered [dA] and [dT] stretches were located nearly in phase with the DNA helical screw. One of the DNA segments characterized in this study appeared to be derived from the giant linear plasmid, SCP1, and another from the circular fertility plasmid, SCP2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-1369
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Primary characterization of curved DNA segments cloned from Streptomyces DNA with extremely high G/C-contents.
pubmed:affiliation
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't