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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1991-7-3
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pubmed:abstractText |
Oligosaccharides produced during the course of the hydrolysis of 25% N-acetylated chitosan by Streptomyces griseus chitinase were fractionated by CM-Sephadex C-25 and Toyopearl HW-40F column chromatographies. Sugar compositions and sequences of main oligosaccharides were identified by N-acetylation, exo-splitting with beta-GlcNAcase and beta-GlcNase, and nitrous acid degradation. In addition to N-acetylated saccharides, GlcNAc, (GlcNAc)2, and (GlcNAc)3, hetero-chitooligosaccharides such as GlcN.GlcNAc, GlcN.GlcNAc.GlcNAc, GlcN.GlcN.GlcNAc, GlcN.GlcNAc.GlcNAc.GlcNAc, GlcNAc.GlcN.GlcNAc.GlcNAc, GlcN.GlcNAc.GlcN.GlcNAc, and GlcN.GlcN.GlcNAc.GlcNAc were identified. These results indicate that Streptomyces griseus chitinase specifically cleaves the N-acetyl-beta-D-glucosaminidic linkages in partially N-acetylated chitosan.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
B
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chitin,
http://linkedlifedata.com/resource/pubmed/chemical/Chitinase,
http://linkedlifedata.com/resource/pubmed/chemical/Chitosan,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrous Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0002-1369
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3191-9
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:1368638-Acetylation,
pubmed-meshheading:1368638-Carbohydrate Sequence,
pubmed-meshheading:1368638-Chitin,
pubmed-meshheading:1368638-Chitinase,
pubmed-meshheading:1368638-Chitosan,
pubmed-meshheading:1368638-Chromatography, High Pressure Liquid,
pubmed-meshheading:1368638-Hydrolysis,
pubmed-meshheading:1368638-Molecular Sequence Data,
pubmed-meshheading:1368638-Nitrous Acid,
pubmed-meshheading:1368638-Oligosaccharides,
pubmed-meshheading:1368638-Streptomyces griseus
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pubmed:year |
1990
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pubmed:articleTitle |
Action pattern of Streptomyces griseus chitinase on partially N-acetylated chitosan.
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pubmed:affiliation |
Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Japan.
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pubmed:publicationType |
Journal Article
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