Linked Life Data

1366900

Source:http://linkedlifedata.com/resource/pubmed/id/1366900

Statements in which the resource exists.
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pubmed-article:1366900pubmed:abstractTextWe transformed Aspergillus niger with the full length cDNA gene encoding hen egg-white lysozyme (HEWL) and its secretion signal sequence. Lysozyme levels up to 12 mg/l were secreted when expression was controlled by the A. awamori glucoamylase (GAM) promoter and 1 mg/l when controlled by the A. nidulans glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter. N-terminal sequence analysis of the recombinant protein indicated that the signal peptide was correctly processed by the A. niger secretory apparatus. The specific catalytic activity of the recombinant protein was identical to that of authentic hen lysozyme. The recombinant HEWL was examined by 2D 1H-NMR spectroscopy and shown to have a spectrum identical to that of authentic HEWL indicating that the protein was correctly folded.lld:pubmed
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pubmed-article:1366900pubmed:articleTitleHen egg white lysozyme expressed in, and secreted from, Aspergillus niger is correctly processed and folded.lld:pubmed
pubmed-article:1366900pubmed:affiliationAgricultural and Food Research Council Institute of Food Research, Norwich Laboratory, U.K.lld:pubmed
pubmed-article:1366900pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1366900pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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