| pubmed-article:1312566 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1312566 | lifeskim:mentions | umls-concept:C1123023 | lld:lifeskim |
| pubmed-article:1312566 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1312566 | lifeskim:mentions | umls-concept:C0034785 | lld:lifeskim |
| pubmed-article:1312566 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:1312566 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1312566 | pubmed:dateCreated | 1992-4-23 | lld:pubmed |
| pubmed-article:1312566 | pubmed:abstractText | Radioligand-binding experiments were performed with crude membrane homogenates (CMH) from human skin in order to investigate the epidermal beta-adrenergic receptor (beta AR) density. CMH were prepared from leftovers of split-thickness skin grafts by sequential homogenization and centrifugation procedures to yield essentially epidermal fragments. Saturation experiments with the non-selective beta-adrenoceptor antagonist (-)-[125I]-iodocyanopindolol (ICYP) as radioligand showed saturable specific binding isotherms. Scatchard transformation of the data demonstrated high-affinity binding of ICYP to a single class of beta AR (Bmax = 80 +/- 10 fmol/mg protein; KD = 8 pM +/- 0.9; n = 8). beta AR antagonists displaced ICYP in a monophasic displacement pattern. The IC50 values were (nmol/1) propranolol (non-selective) 24.8; ICI 118,551 (beta 2 selective) 14.7; CGP-12177 (non-selective) 28.9; bisoprolol (beta 1 selective) 1500; CGP-20712A (beta 1 selective) 8990. beta AR agonists displaced ICYP with a potency ranking isoprenaline greater than adrenaline greater than noradrenaline. We conclude that epidermal crude membrane homogenates prepared from human split-thickness skin contain a high population of beta 2-adrenergic receptors. These receptors may be studied to further investigate the nature of human epidermal beta-adrenergic mechanisms. | lld:pubmed |
| pubmed-article:1312566 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1312566 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1312566 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1312566 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1312566 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1312566 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1312566 | pubmed:issn | 0022-202X | lld:pubmed |
| pubmed-article:1312566 | pubmed:author | pubmed-author:SteinfathMM | lld:pubmed |
| pubmed-article:1312566 | pubmed:author | pubmed-author:MensingHH | lld:pubmed |
| pubmed-article:1312566 | pubmed:author | pubmed-author:KörnerCC | lld:pubmed |
| pubmed-article:1312566 | pubmed:author | pubmed-author:SteinkrausVV | lld:pubmed |
| pubmed-article:1312566 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1312566 | pubmed:volume | 98 | lld:pubmed |
| pubmed-article:1312566 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1312566 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1312566 | pubmed:pagination | 475-80 | lld:pubmed |
| pubmed-article:1312566 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:meshHeading | pubmed-meshheading:1312566-... | lld:pubmed |
| pubmed-article:1312566 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1312566 | pubmed:articleTitle | Binding of beta-adrenergic receptors in human skin. | lld:pubmed |
| pubmed-article:1312566 | pubmed:affiliation | Department of Dermatology, University of Hamburg, Germany. | lld:pubmed |
| pubmed-article:1312566 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1312566 | lld:pubmed |
