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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2003-12-5
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pubmed:abstractText |
The interaction of osteopontin (OPN) with CD44 and alphavbeta3-integrin has been implicated in numerous signal transduction pathways that may promote cancer metastasis. CD44v6 is a splice variant of CD44 which has been identified as a marker of cancer progression. In this study, immortalized liver carcinoma cells (HepG2) were used to examine the effect of OPN on two isoforms of CD44: CD44 standard (CD44 s) and CD44v6. Western blots demonstrated that OPN up-regulated plasma membrane CD44v6 protein expression in a concentration- and time-dependent fashion. CD44v6 levels returned to control levels when OPN-alphavbeta3-integrin binding was blocked by an RGD peptide or tyrosine kinase activity was inhibited. OPN significantly increased CD44v6 protein synthesis, while simultaneously decreasing protein degradation. Steady-state mRNA levels of both CD44s and CD44v6 were unaltered in the presence of OPN stimulation. OPN increased HepG2 in vitro adhesion to hyaluronate (HA); excess soluble HA extinguished OPN-mediated HepG2 adhesion, indicating CD44 dependence. In conclusion, OPN binds to the alphavbeta3-integrin to increase plasma membrane CD44v6 expression and augment in vitro adhesion to HA. This may contribute to the mechanism by which OPN enhances metastatic behavior in hepatocellular cancer cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/CD44v6 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Combinations,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alphaVbeta3,
http://linkedlifedata.com/resource/pubmed/chemical/Laminin,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Osteopontin,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SPP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/arginyl-glycyl-aspartic acid,
http://linkedlifedata.com/resource/pubmed/chemical/matrigel
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0143-3334
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1871-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12949055-Antigens, CD44,
pubmed-meshheading:12949055-Blotting, Northern,
pubmed-meshheading:12949055-Blotting, Western,
pubmed-meshheading:12949055-Carcinoma, Hepatocellular,
pubmed-meshheading:12949055-Cell Adhesion,
pubmed-meshheading:12949055-Cell Division,
pubmed-meshheading:12949055-Cell Line,
pubmed-meshheading:12949055-Cell Movement,
pubmed-meshheading:12949055-Collagen,
pubmed-meshheading:12949055-Dose-Response Relationship, Drug,
pubmed-meshheading:12949055-Drug Combinations,
pubmed-meshheading:12949055-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12949055-Glycoproteins,
pubmed-meshheading:12949055-Humans,
pubmed-meshheading:12949055-Hyaluronic Acid,
pubmed-meshheading:12949055-Immunoblotting,
pubmed-meshheading:12949055-Integrin alphaVbeta3,
pubmed-meshheading:12949055-Laminin,
pubmed-meshheading:12949055-Oligopeptides,
pubmed-meshheading:12949055-Osteopontin,
pubmed-meshheading:12949055-Protein Isoforms,
pubmed-meshheading:12949055-Proteoglycans,
pubmed-meshheading:12949055-RNA,
pubmed-meshheading:12949055-RNA, Messenger,
pubmed-meshheading:12949055-Sialoglycoproteins,
pubmed-meshheading:12949055-Signal Transduction,
pubmed-meshheading:12949055-Time Factors,
pubmed-meshheading:12949055-Up-Regulation
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pubmed:year |
2003
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pubmed:articleTitle |
Osteopontin-dependent CD44v6 expression and cell adhesion in HepG2 cells.
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pubmed:affiliation |
Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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