Linked Life Data

12941158

Source:http://linkedlifedata.com/resource/pubmed/id/12941158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-8-28
pubmed:abstractText
The process of creating chimeric mRNA transcripts derived from separately transcribed genes via known spliceosome mechanisms is termed trans-splicing, and has been primarily described in lower eukaryotes. Isolation of cDNA clones containing sequences from distinct genes has raised the possibility of trans-splicing across distinct genes in mammalian systems; however, the possibility of cloning artifacts or splicing via nonspliceosome mechanisms has been difficult to rule out. In most cases, the absence of corresponding genomic clones has limited assessment of splice donor and acceptance sites and associated intronic elements that would be expected to participate in spliceosome-based reactions. We have previously reported a cDNA clone encoding the rat Leukocyte Common Antigen-Related (LAR) tyrosine phosphatase receptor that contains an alternative 3' UTR. In the present study Northern, RT-PCR and RNase protection assays verified the existence of developmentally regulated 3' UTR alternative splicing of LAR transcripts in vivo. FISH and radiation hybrid mapping demonstrated that loci encoding LAR and its alternative 3' UTR are present on distinct chromosomes, raising the possibility that alternatively spliced transcripts resulted from trans-splicing. Exon/intron analysis of corresponding genomic clones revealed nonconsensus splice junctions along with elements known to promote both cis- and trans-splicing. Verification in a mammalian in vivo system of chimeric transcripts derived from distinct genes along with identification of atypical nonconsensus-associated genomic elements points to the novel possibilities of atypical spliceosome-based trans-splicing or nonconventional, nonspliceosome-based mechanisms leading to chimeric transcripts.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
303-15
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12941158-3' Untranslated Regions, pubmed-meshheading:12941158-Alternative Splicing, pubmed-meshheading:12941158-Animals, pubmed-meshheading:12941158-Base Sequence, pubmed-meshheading:12941158-Blotting, Northern, pubmed-meshheading:12941158-Chimera, pubmed-meshheading:12941158-Chromosomes, pubmed-meshheading:12941158-Conserved Sequence, pubmed-meshheading:12941158-DNA, Complementary, pubmed-meshheading:12941158-DNA Primers, pubmed-meshheading:12941158-Exons, pubmed-meshheading:12941158-Gene Expression Regulation, Developmental, pubmed-meshheading:12941158-Genome, pubmed-meshheading:12941158-Introns, pubmed-meshheading:12941158-Molecular Sequence Data, pubmed-meshheading:12941158-Nerve Tissue Proteins, pubmed-meshheading:12941158-Protein Tyrosine Phosphatases, pubmed-meshheading:12941158-RNA, Messenger, pubmed-meshheading:12941158-Radiation Hybrid Mapping, pubmed-meshheading:12941158-Rats, pubmed-meshheading:12941158-Rats, Sprague-Dawley, pubmed-meshheading:12941158-Receptor-Like Protein Tyrosine Phosphatases, Class 2, pubmed-meshheading:12941158-Receptors, Cell Surface, pubmed-meshheading:12941158-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12941158-Ribonuclease, Pancreatic, pubmed-meshheading:12941158-Trans-Splicing
pubmed:year
2003
pubmed:articleTitle
A candidate chimeric mammalian mRNA transcript is derived from distinct chromosomes and is associated with nonconsensus splice junction motifs.
pubmed:affiliation
Department of Neurology, University of California at San Francisco, San Francisco, California, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't