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rdf:type |
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lifeskim:mentions |
umls-concept:C0007595,
umls-concept:C0009015,
umls-concept:C0017262,
umls-concept:C0035820,
umls-concept:C0185117,
umls-concept:C0333117,
umls-concept:C0596138,
umls-concept:C0699040,
umls-concept:C1167622,
umls-concept:C1519249,
umls-concept:C1880022,
umls-concept:C2911684
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pubmed:issue |
44
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pubmed:dateCreated |
2003-10-27
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pubmed:databankReference |
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pubmed:abstractText |
Cell surface retention sequence binding protein-1 (CRSBP-1) is a cell surface binding protein for the cell surface retention sequence (CRS) motif of the v-sis gene product (platelet-derived growth factor-BB). It has been shown to be responsible for cell surface retention of the v-sis gene product in v-sis-transformed cells (fibroblasts) and has been hypothesized to play a role in autocrine growth and transformation of these cells. Here we demonstrate that the CRSBP-1 cDNA cloned from bovine liver libraries encodes a 322-residue type I membrane protein containing a 23-residue signal peptide, a 215-residue cell surface domain, a 21-residue transmembrane domain, and a 63-residue cytoplasmic domain. CRSBP-1 expressed in transfected cells is an approximately 120-kDa disulfide-linked homodimeric glycoprotein and exhibits dual ligand (CRS-containing growth regulators (v-sis gene product and insulin-like growth factor binding protein-3, IGFBP-3) and hyaluronic acid) binding activity. CRSBP-1 overexpression (by stable transfection of cells with CRSBP-1 cDNA) enhances autocrine loop signaling, cell growth, and tumorigenicity (in mice) of v-sis-transformed cells. CRSBP-1 expression also enhances autocrine cell growth mediated by IGFBP-3 in human lung carcinoma cells (H1299 cells), which express very little, if any, endogenous CRSBP-1 and exhibits a mitogenic response to exogenous IGFBP-3, stably transfected with IGFBP-3 cDNA. However, CRSBP-1 overexpression does not affect growth of normal and transformed cells that do not produce these CRS-containing growth regulators. These results suggest that CRSBP-1 plays a role in autocrine regulation of cell growth mediated by growth regulators containing CRS.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Detergents,
http://linkedlifedata.com/resource/pubmed/chemical/Disulfides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octoxynol,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins v-sis,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Sorting Signals,
http://linkedlifedata.com/resource/pubmed/chemical/cell-surface retention-binding...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43855-69
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12912978-Amino Acid Motifs,
pubmed-meshheading:12912978-Amino Acid Sequence,
pubmed-meshheading:12912978-Animals,
pubmed-meshheading:12912978-Base Sequence,
pubmed-meshheading:12912978-Blotting, Western,
pubmed-meshheading:12912978-Cattle,
pubmed-meshheading:12912978-Cell Division,
pubmed-meshheading:12912978-Cell Line, Transformed,
pubmed-meshheading:12912978-Cell Line, Tumor,
pubmed-meshheading:12912978-Cytoplasm,
pubmed-meshheading:12912978-DNA, Complementary,
pubmed-meshheading:12912978-Detergents,
pubmed-meshheading:12912978-Dimerization,
pubmed-meshheading:12912978-Disulfides,
pubmed-meshheading:12912978-Fibroblasts,
pubmed-meshheading:12912978-Gene Library,
pubmed-meshheading:12912978-Glycoproteins,
pubmed-meshheading:12912978-Humans,
pubmed-meshheading:12912978-Immunoblotting,
pubmed-meshheading:12912978-Insulin-Like Growth Factor Binding Protein 3,
pubmed-meshheading:12912978-Liver,
pubmed-meshheading:12912978-Membrane Proteins,
pubmed-meshheading:12912978-Mice,
pubmed-meshheading:12912978-Molecular Sequence Data,
pubmed-meshheading:12912978-NIH 3T3 Cells,
pubmed-meshheading:12912978-Octoxynol,
pubmed-meshheading:12912978-Oncogene Proteins v-sis,
pubmed-meshheading:12912978-Peptides,
pubmed-meshheading:12912978-Protein Binding,
pubmed-meshheading:12912978-Protein Sorting Signals,
pubmed-meshheading:12912978-Sequence Homology, Amino Acid,
pubmed-meshheading:12912978-Time Factors,
pubmed-meshheading:12912978-Transfection
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pubmed:year |
2003
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pubmed:articleTitle |
Cloning, expression, characterization, and role in autocrine cell growth of cell surface retention sequence binding protein-1.
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pubmed:affiliation |
Departments of Biochemistry and Molecular Biology, St. Louis University School of Medicine, St. Louis, Missouri 63104, USA. huangjs@slu.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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