Linked Life Data

12903118

Source:http://linkedlifedata.com/resource/pubmed/id/12903118

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-8-7
pubmed:abstractText
We have studied the mutagenic specificity of abasic sites using the yeast oligonucleotides transformation assay. We introduced oligonucleotide containing a natural abasic site and a tetrahydrofuran abasic site into Rev1 mutants, rev1AA, which contains mutations of Asp467 and Glu468 residues of Rev1p to Ala in order to inactivate dCMP transferase activity, and rev1 delta, which lacks its whole coding sequence. The transformation efficiencies of rev1AA with abasic-containing oligonucleotides were lower than those of B7528, a strain proficient in REV1 gene, but much higher than rev1 delta mutant. Sequence analysis opposite the lesions showed that the mutation spectra were different among these three strains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:author
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
87-8
pubmed:dateRevised
2006-11-30
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
The role of deoxycytidyl transferase activity of yeast Rev1 protein in the bypass of abasic sites.
pubmed:affiliation
Gene Research Center, Okayama University, Tsushima, Okayama 700-8530, Japan.
pubmed:publicationType
Journal Article