Linked Life Data

12894843

Source:http://linkedlifedata.com/resource/pubmed/id/12894843

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2003-8-4
pubmed:abstractText
For the analysis of dioxins, polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) and coplanar polychlorinated biphenyls (Co-PCBs), devising simple and economical methods is important, especially for mass screening of human exposure. Pretreatment of samples, namely the extraction and cleanup methods that are widely used at present, needs to be improved for savings in time, manpower, and solvent consumption. In the present study, we applied solid phase extraction (SPE) using octadecyl (C18) and a blue-chitin column in place of liquid-liquid extraction (LE) and an active-carbon column with serum samples, frequently used for assessment of human exposure. Efficacy of the new pretreatment methods was demonstrated by successful high resolution gas chromatography-high resolution mass spectrometry (HRGC-HRMS) of the major 17 PCDDs/Fs and 12 Co-PCBs that are on the list of WHO/IPCS (1997) hazardous dioxins with toxic equivalent factor (TEF) values. SPE is timesaving and requires less manpower and organic solvent as compared with the LE that is presently widely used. Concerning cleanup with blue-chitin, the amount of toluene applied as eluent could be reduced to 1/3, as compared with the active-carbon case. The combination of SPE and blue-chitin for pretreatment of serum saves time and manpower, is accurate and uses less organic solvent than LE with active carbon cleanup.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-2654
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
986-93
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Effective pretreatment of human serum samples for dioxin analysis by solid phase extraction and blue-chitin column cleanup.
pubmed:affiliation
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't