Linked Life Data

12876343

Source:http://linkedlifedata.com/resource/pubmed/id/12876343

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 8
pubmed:dateCreated
2003-7-23
pubmed:abstractText
MrsD from Bacillus sp. HIL-Y85/54728 is a member of the HFCD (homo-oligomeric flavin-containing Cys decarboxylases) family of flavoproteins and is involved in the biosynthesis of the lantibiotic mersacidin. It catalyses the oxidative decarboxylation of the C-terminal cysteine residue of the MrsA precursor peptide of mersacidin, yielding a (Z)-enethiol intermediate as the first step in the formation of the unusual amino acid S-[(Z)-2-aminovinyl]-methyl-D-cysteine. Surprisingly, MrsD was found to bind FAD, in contrast to the three other characterized members of the HFCD family, which bind FMN. To determine the molecular discriminators of FAD binding within the HFCD family, the crystal structure of MrsD was analyzed at a resolution of 2.54 A. Crystals of space group F432 contain one MrsD monomer in the asymmetric unit. However, a Patterson search with EpiD-derived models failed. Based on the consideration that the dodecameric MrsD particle of tetrahedral symmetry resembles the quaternary structure of EpiD, rotational and translational parameters were derived from the geometric consideration that the MrsD dodecamer is generated from a monomer by crystallographic symmetry around the position (1/4, 1/4, 1/4) of the unit cell. A structural comparison with the FMN-binding members of the HFCD family EpiD and AtHAL3a shows conserved sequence motifs in contact with the flavin's pyrimidine ring but divergent environments for the dimethylbenzene ring of the isoalloxazine moiety. The position of the ribityl chain differs in MrsD from that found in EpiD and AtHAL3a. However, the FMN-phosphate binding sites are also highly conserved in their exact positions. In all three cases, the flavin cofactor is bound to a structurally conserved region of the Rossmann-fold monomer, exposing its Re side for catalysis. The adenosyl phosphate of FAD is anchored in a well defined binding site and the adenosine moieties are oriented towards the interior of the hollow particle, where three of them pack against each other around the threefold axis of a trimeric facet.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0907-4449
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1414-21
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed-meshheading:12876343-Bacterial Proteins, pubmed-meshheading:12876343-Bacteriocins, pubmed-meshheading:12876343-Binding Sites, pubmed-meshheading:12876343-Carboxy-Lyases, pubmed-meshheading:12876343-Catalysis, pubmed-meshheading:12876343-Crystallography, X-Ray, pubmed-meshheading:12876343-Cysteine, pubmed-meshheading:12876343-Electrons, pubmed-meshheading:12876343-Escherichia coli, pubmed-meshheading:12876343-Flavin-Adenine Dinucleotide, pubmed-meshheading:12876343-Flavins, pubmed-meshheading:12876343-Flavoproteins, pubmed-meshheading:12876343-Models, Chemical, pubmed-meshheading:12876343-Models, Molecular, pubmed-meshheading:12876343-Oxygen, pubmed-meshheading:12876343-Peptides, pubmed-meshheading:12876343-Protein Binding, pubmed-meshheading:12876343-Protein Structure, Quaternary, pubmed-meshheading:12876343-Protein Structure, Tertiary
pubmed:year
2003
pubmed:articleTitle
Structure of MrsD, an FAD-binding protein of the HFCD family.
pubmed:affiliation
Max-Planck-Institut für Biochemie, Abteilung für Strukturforschung, Am Klopferspitz 18a, D-82152 Martinsried, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't