Linked Life Data

12842861

Source:http://linkedlifedata.com/resource/pubmed/id/12842861

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-7-4
pubmed:abstractText
Data suggest that mineralocorticoid selectivity is differentially regulated in epithelial target tissues. We investigated whether the level of dietary NaCl intake influenced the expression and tissue distribution of 11-beta-hydroxysteroid dehydrogenase type 2 (11betaHSD-2), aldosterone receptor (MR), and glucocorticoid receptor (GR) in rat colon, kidney, and cardiovascular tissue. Rats were fed a diet with 0.01 or 3% NaCl for 10 days. Messenger RNAs were analyzed with ribonuclease protection assay, 11betaHSD-2 protein by Western blot analysis, and localization of GR and 11betaHSD-2 by immunohistochemistry. NaCl restriction elevated plasma renin and aldosterone concentration, whereas corticosterone was unaltered. In distal colon, 11betaHSD-2 mRNA and protein were augmented significantly by low-NaCl intake and immunolabeling was widely distributed in crypt and surface epithelium. The MR mRNA level was decreased, whereas GR mRNA was unaltered in distal colon. MR, GR, and 11betaHSD-2 mRNAs were not changed in kidney cortex and medulla, left cardiac ventricle, and aorta. Immunofluorescence labeling showed that GR and 11betaHSD-2 localization was mutually exclusive in kidney. In colon epithelium, nuclear staining for GR subsided as perinuclear 11betaHSD-2 immunoreactivity increased with NaCl restriction. As a functional correlate of increased 11betaHSD-2 expression in colon, the GR-stimulated sodium-hydrogen exchanger NHE-3 was lowered by NaCl restriction. Inhibition of 11betaHSD-2 activity by carbenoxolone during NaCl restriction stimulated NHE-3 expression in colon. Dexamethasone stimulated NHE-3 both in colon and kidney. These data indicate that mineralocorticoid selectivity is physiologically regulated by NaCl intake at the level of 11betaHSD-2 expression and tissue distribution in the distal colon, but not in the kidney.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1931-857X
pubmed:author
pubmed:issnType
Print
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F348-58
pubmed:dateRevised
2011-4-28
pubmed:meshHeading
pubmed-meshheading:12842861-11-beta-Hydroxysteroid Dehydrogenase Type 2, pubmed-meshheading:12842861-Aldosterone, pubmed-meshheading:12842861-Animals, pubmed-meshheading:12842861-Cardiovascular System, pubmed-meshheading:12842861-Colon, pubmed-meshheading:12842861-Corticosterone, pubmed-meshheading:12842861-Epithelial Cells, pubmed-meshheading:12842861-Gene Expression, pubmed-meshheading:12842861-Gene Expression Regulation, Enzymologic, pubmed-meshheading:12842861-Hydroxysteroid Dehydrogenases, pubmed-meshheading:12842861-Kidney Cortex, pubmed-meshheading:12842861-Male, pubmed-meshheading:12842861-Rats, pubmed-meshheading:12842861-Rats, Sprague-Dawley, pubmed-meshheading:12842861-Receptors, Glucocorticoid, pubmed-meshheading:12842861-Receptors, Mineralocorticoid, pubmed-meshheading:12842861-Renin, pubmed-meshheading:12842861-Sodium Chloride, Dietary
pubmed:year
2003
pubmed:articleTitle
Stimulation of 11-beta-hydroxysteroid dehydrogenase type 2 in rat colon but not in kidney by low dietary NaCl intake.
pubmed:affiliation
Department of Physiology and Pharmacology, University of Southern Denmark, Winsloewparken 21, 3 DK-5000 Odense C, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't