| pubmed-article:1282493 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1282493 | lifeskim:mentions | umls-concept:C0036636 | lld:lifeskim |
| pubmed-article:1282493 | lifeskim:mentions | umls-concept:C0282580 | lld:lifeskim |
| pubmed-article:1282493 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:1282493 | lifeskim:mentions | umls-concept:C0003261 | lld:lifeskim |
| pubmed-article:1282493 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:1282493 | lifeskim:mentions | umls-concept:C1704419 | lld:lifeskim |
| pubmed-article:1282493 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:1282493 | pubmed:dateCreated | 1993-2-10 | lld:pubmed |
| pubmed-article:1282493 | pubmed:abstractText | The rational development of peptide vaccines requires the identification of both B- and T-cell epitopes. In this study, potential T-helper cell epitopes of Semliki Forest virus (SFV) were identified on the basis of their ability to induce delayed-type hypersensitivity (DTH) in mice using recombinant SFV fragments produced as hybrid proteins with beta-galactosidase in Escherichia coli and synthetic peptides coupled to beta-galactosidase. Although the tested fragments spanned almost the entire amino acid sequence of the structural proteins of SFV, only one DTH-inducing region (located between amino acid 137 and 151 of the SFV E2 membrane protein) was identified. Peptides containing this E2 region stimulated lymph node cells from SFV-primed mice in vitro. The ability of the identified T-cell epitope to induce a specific T-helper response in mice was evaluated using synthetic peptides that contained combinations of the DTH-inducing region and different previously identified linear B-cell epitopes of E2. These peptides proved able to induce an antipeptide IgG response in mice in an H-2d-restricted fashion. One of the peptides was also able to induce high titres of IgG reactive with SFV-infected cells and protected 70-100% of the peptide-immunized mice after challenge with virulent SFV. Our findings suggest that DTH and T-helper activity are mediated by different doses of the same T-cell epitope. | lld:pubmed |
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| pubmed-article:1282493 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1282493 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1282493 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1282493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1282493 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1282493 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:1282493 | pubmed:issn | 0019-2805 | lld:pubmed |
| pubmed-article:1282493 | pubmed:author | pubmed-author:SnippeHH | lld:pubmed |
| pubmed-article:1282493 | pubmed:author | pubmed-author:FernandesRR | lld:pubmed |
| pubmed-article:1282493 | pubmed:author | pubmed-author:KraaijeveldC... | lld:pubmed |
| pubmed-article:1282493 | pubmed:author | pubmed-author:SnijdersAA | lld:pubmed |
| pubmed-article:1282493 | pubmed:author | pubmed-author:Benaissa-Trou... | lld:pubmed |
| pubmed-article:1282493 | pubmed:author | pubmed-author:Visser-Vernoo... | lld:pubmed |
| pubmed-article:1282493 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1282493 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:1282493 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1282493 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1282493 | pubmed:pagination | 322-9 | lld:pubmed |
| pubmed-article:1282493 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
| pubmed-article:1282493 | pubmed:meshHeading | pubmed-meshheading:1282493-... | lld:pubmed |
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| pubmed-article:1282493 | pubmed:meshHeading | pubmed-meshheading:1282493-... | lld:pubmed |
| pubmed-article:1282493 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1282493 | pubmed:articleTitle | A delayed-type hypersensitivity-inducing T-cell epitope of Semliki Forest virus mediates effective T-helper activity for antibody production. | lld:pubmed |
| pubmed-article:1282493 | pubmed:affiliation | Eijkman-Winkler Laboratory of Medical Microbiology, Academic Hospital Utrecht, The Netherlands. | lld:pubmed |
| pubmed-article:1282493 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1282493 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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