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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1993-2-10
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pubmed:abstractText |
The rational development of peptide vaccines requires the identification of both B- and T-cell epitopes. In this study, potential T-helper cell epitopes of Semliki Forest virus (SFV) were identified on the basis of their ability to induce delayed-type hypersensitivity (DTH) in mice using recombinant SFV fragments produced as hybrid proteins with beta-galactosidase in Escherichia coli and synthetic peptides coupled to beta-galactosidase. Although the tested fragments spanned almost the entire amino acid sequence of the structural proteins of SFV, only one DTH-inducing region (located between amino acid 137 and 151 of the SFV E2 membrane protein) was identified. Peptides containing this E2 region stimulated lymph node cells from SFV-primed mice in vitro. The ability of the identified T-cell epitope to induce a specific T-helper response in mice was evaluated using synthetic peptides that contained combinations of the DTH-inducing region and different previously identified linear B-cell epitopes of E2. These peptides proved able to induce an antipeptide IgG response in mice in an H-2d-restricted fashion. One of the peptides was also able to induce high titres of IgG reactive with SFV-infected cells and protected 70-100% of the peptide-immunized mice after challenge with virulent SFV. Our findings suggest that DTH and T-helper activity are mediated by different doses of the same T-cell epitope.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-1656276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-1694039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-1706409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-1997399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2115551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2409149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2435793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2443856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2452085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2461306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2472448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2476243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2523712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2717617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-2953788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-311341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-5123325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-6174604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-6224879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-6316848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-66195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1282493-6985476
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
322-9
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1282493-Animals,
pubmed-meshheading:1282493-Antibodies, Viral,
pubmed-meshheading:1282493-Antigens, Viral,
pubmed-meshheading:1282493-Cell Division,
pubmed-meshheading:1282493-Epitopes,
pubmed-meshheading:1282493-Hypersensitivity, Delayed,
pubmed-meshheading:1282493-Immunization,
pubmed-meshheading:1282493-Immunoglobulin G,
pubmed-meshheading:1282493-Lymph Nodes,
pubmed-meshheading:1282493-Mice,
pubmed-meshheading:1282493-Mice, Inbred Strains,
pubmed-meshheading:1282493-Peptide Fragments,
pubmed-meshheading:1282493-Semliki forest virus,
pubmed-meshheading:1282493-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:1282493-Togaviridae Infections
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pubmed:year |
1992
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pubmed:articleTitle |
A delayed-type hypersensitivity-inducing T-cell epitope of Semliki Forest virus mediates effective T-helper activity for antibody production.
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pubmed:affiliation |
Eijkman-Winkler Laboratory of Medical Microbiology, Academic Hospital Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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