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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0018270,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0027651,
umls-concept:C0031437,
umls-concept:C0034703,
umls-concept:C0185117,
umls-concept:C0205359,
umls-concept:C0443211,
umls-concept:C0751690,
umls-concept:C1510411,
umls-concept:C1548328,
umls-concept:C1553412,
umls-concept:C1880022,
umls-concept:C2246239,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
2003-8-11
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pubmed:abstractText |
Transplantable tumor (KE) and clone cell (KE-F11) lines were established from a spontaneous malignant schwannoma found in an aged F344 rat. The primary tumor and KE tumors consisted of oval or spindle cells arranged in ill-defined bundles. Cultured KE-F11 cells exhibited polygonal or spindle configurations. Immunohistochemically, neoplastic cells in KE and KE-F11 reacted to vimentin, S-100 protein, neuron-specific enolase, myelin basic protein, and glial fibrillary acidic protein in varying degrees, indicating neurogenic features; occasional cells reacted to alpha-smooth muscle actin. Cells positive for lysosomal enzymes (acid phosphatase and non-specific esterase), and ED1 (rat macrophage specific) were observed in KE-F11, and electron microscopically, cells with many lysosomes were frequently present, indicating expression of macrophage-like phenotypes. Bioassay analysis revealed that KE-F11 cells produced high levels of nerve growth factor. DNA synthesis was inhibited by addition of transforming growth factor-beta1 (TGF-beta1), and Northern blot analysis revealed that expression of c-myc, a cell cycle-related immediate early gene, was depressed by TGF-beta1. Likely, TGF-beta1 is a factor capable of inhibiting cellular growth of Schwann cells. mRNA expression of the low-density lipoprotein receptor-related protein (LRP) was seen in KE-F11 cells by Northern blot analysis, and the level was decreased by lipopolysaccharide (LPS) treatment. LRP may be attributable to regulation of Schwann cell functions. KE-F11 cells seeded on laminin-coated dishes exhibited more extended cytoplasmic projections than on collagen type I-coated dishes. The present study provides evidence that biological properties of malignant schwannoma-derived cells might be affected by exogenous factors such as TGF-beta1, LPS and laminin. These tumor lines may be useful for studies on pathobiological characteristics of Schwann cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Desmin,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Keratins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Myelin Basic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphopyruvate Hydratase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1,
http://linkedlifedata.com/resource/pubmed/chemical/Vimentin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0001-6322
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
221-33
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pubmed:dateRevised |
2007-11-9
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pubmed:meshHeading |
pubmed-meshheading:12811582-Actins,
pubmed-meshheading:12811582-Animals,
pubmed-meshheading:12811582-Blotting, Northern,
pubmed-meshheading:12811582-Cell Count,
pubmed-meshheading:12811582-Cell Line, Transformed,
pubmed-meshheading:12811582-Desmin,
pubmed-meshheading:12811582-Dose-Response Relationship, Drug,
pubmed-meshheading:12811582-Genes, jun,
pubmed-meshheading:12811582-Genes, myc,
pubmed-meshheading:12811582-Glial Fibrillary Acidic Protein,
pubmed-meshheading:12811582-Keratins,
pubmed-meshheading:12811582-Lipopolysaccharides,
pubmed-meshheading:12811582-Macrophages,
pubmed-meshheading:12811582-Male,
pubmed-meshheading:12811582-Microscopy, Electron,
pubmed-meshheading:12811582-Myelin Basic Proteins,
pubmed-meshheading:12811582-Nerve Growth Factor,
pubmed-meshheading:12811582-Neurilemmoma,
pubmed-meshheading:12811582-PC12 Cells,
pubmed-meshheading:12811582-Phenotype,
pubmed-meshheading:12811582-Phosphopyruvate Hydratase,
pubmed-meshheading:12811582-RNA, Messenger,
pubmed-meshheading:12811582-Rats,
pubmed-meshheading:12811582-Rats, Inbred F344,
pubmed-meshheading:12811582-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12811582-S100 Proteins,
pubmed-meshheading:12811582-Staining and Labeling,
pubmed-meshheading:12811582-Time Factors,
pubmed-meshheading:12811582-Transforming Growth Factor beta,
pubmed-meshheading:12811582-Transforming Growth Factor beta1,
pubmed-meshheading:12811582-Vimentin
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pubmed:year |
2003
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pubmed:articleTitle |
Characterization of newly established tumor lines from a spontaneous malignant schwannoma in F344 rats: nerve growth factor production, growth inhibition by transforming growth factor-beta1, and macrophage-like phenotype expression.
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pubmed:affiliation |
Laboratories of Veterinary Pathology and Anatomy, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Gakuencho 1-1, Sakai, 599-8531 Osaka, Japan. yamate@vet.osakafu-u.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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