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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2003-6-13
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pubmed:abstractText |
C-terminal-binding protein/brefeldin A-ADP ribosylated substrate (CtBP/BARS) plays key roles in development and oncogenesis as a transcription co-repressor, and in intracellular traffic as a promoter of Golgi membrane fission. Co-repressor activity is regulated by NAD(H) binding to CtBP/BARS, while membrane fission is associated with its acyl-CoA-dependent acyltransferase activity. Here, we report the crystal structures of rat CtBP/BARS in a binary complex with NAD(H), and in a ternary complex with a PIDLSKK peptide mimicking the consensus motif (PXDLS) recognized in CtBP/BARS cellular partners. The structural data show CtBP/BARS in a NAD(H)-bound dimeric form; the peptide binding maps the recognition site for DNA-binding proteins and histone deacetylases to an N-terminal region of the protein. The crystal structure together with the site-directed mutagenesis data and binding experiments suggest a rationale for the molecular mechanisms underlying the two fundamental co-existing, but diverse, activities supported by CtBP/BARS in the nucleus and in Golgi membranes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-10089316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-10089361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-10364211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-10369679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-10586885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-10592235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-12037320,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-12429083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-12530516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-15299354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-15299374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-1758883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-2025413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-4737475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-7479821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-7624370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-7719856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-7984417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-8114093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-8120891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-8302839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-8440238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-8580836,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-9382860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-9724649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12805226-9757107
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3122-30
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12805226-Acyl Coenzyme A,
pubmed-meshheading:12805226-Amino Acid Sequence,
pubmed-meshheading:12805226-Animals,
pubmed-meshheading:12805226-Binding Sites,
pubmed-meshheading:12805226-Carrier Proteins,
pubmed-meshheading:12805226-Cell Membrane,
pubmed-meshheading:12805226-Crystallography, X-Ray,
pubmed-meshheading:12805226-Golgi Apparatus,
pubmed-meshheading:12805226-Models, Molecular,
pubmed-meshheading:12805226-Molecular Sequence Data,
pubmed-meshheading:12805226-NAD,
pubmed-meshheading:12805226-Peptides,
pubmed-meshheading:12805226-Protein Binding,
pubmed-meshheading:12805226-Protein Folding,
pubmed-meshheading:12805226-Protein Structure, Tertiary,
pubmed-meshheading:12805226-Rats,
pubmed-meshheading:12805226-Recombinant Fusion Proteins,
pubmed-meshheading:12805226-Repressor Proteins,
pubmed-meshheading:12805226-Sequence Alignment,
pubmed-meshheading:12805226-Transcription, Genetic,
pubmed-meshheading:12805226-Transcription Factors
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pubmed:year |
2003
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pubmed:articleTitle |
CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission.
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pubmed:affiliation |
Department of Physics-INFM, University of Genova, Via Dodecaneso 33, 16146 Genova, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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