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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-5-16
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pubmed:abstractText |
GPI-80 is a member of the amidohydrolase family that has been proposed as a potential regulator of beta2-integrin-dependent leukocyte adhesion. GPI-80 is expressed mainly in human neutrophils. Our previous studies suggested that GPI-80 expression might be associated with myeloid differentiation. To verify this, we examined whether GPI-80 is expressed on the human promyelocytic leukemia cell line HL-60 following treatment with differentiation inducers. GPI-80 expression was induced in cells treated with dimethyl sulfoxide (DMSO) to stimulate differentiation down the neutrophil pathway. On the other hand, all-trans-retinoic acid (ATRA), another neutrophil-inducing reagent, induced no clear GPI-80 expression. Potent monocyte-inducing reagents such as 1alpha,25-dihydroxyvitamin D(3) or phorbol 12-myristate 13-acetate also had no significant effect on the protein expression. GPI-80-positive cells were found in the well-differentiated CD11b-positive and transferrin-receptor-negative cell population. Granulocyte colony-stimulating factor, which augments neutrophil differentiation of HL-60 cells, up-regulated GPI-80 expression in the presence of DMSO. Granulocyte/macrophage colony-stimulating factor, which is known to suppress the neutrophil maturation of cells, inhibited expression. Adhesion of DMSO-induced cells was regulated by anti-GPI-80 monoclonal antibody, similar to the regulation observed in neutrophils. These results suggest that use of DMSO to induce neutrophil differentiation provides suitable conditions for GPI-80 expression, and that this culture system may be a helpful model for further study of the regulation of GPI-80 expression during myeloid differentiation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amidohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD11,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Cholecalciferol,
http://linkedlifedata.com/resource/pubmed/chemical/Dimethyl Sulfoxide,
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating...,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transferrin,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/VNN2 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-4827
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
286
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
199-208
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12749849-Amidohydrolases,
pubmed-meshheading:12749849-Antibodies,
pubmed-meshheading:12749849-Antigens, CD11,
pubmed-meshheading:12749849-Antigens, CD18,
pubmed-meshheading:12749849-Cell Adhesion,
pubmed-meshheading:12749849-Cell Adhesion Molecules,
pubmed-meshheading:12749849-Cell Differentiation,
pubmed-meshheading:12749849-Chemotaxis, Leukocyte,
pubmed-meshheading:12749849-Cholecalciferol,
pubmed-meshheading:12749849-Dimethyl Sulfoxide,
pubmed-meshheading:12749849-GPI-Linked Proteins,
pubmed-meshheading:12749849-Gene Expression Regulation,
pubmed-meshheading:12749849-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:12749849-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:12749849-HL-60 Cells,
pubmed-meshheading:12749849-Humans,
pubmed-meshheading:12749849-Hydrolases,
pubmed-meshheading:12749849-Models, Biological,
pubmed-meshheading:12749849-Myeloid Cells,
pubmed-meshheading:12749849-Neutrophils,
pubmed-meshheading:12749849-Receptors, Transferrin,
pubmed-meshheading:12749849-Tetradecanoylphorbol Acetate,
pubmed-meshheading:12749849-Tretinoin
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pubmed:year |
2003
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pubmed:articleTitle |
Expression of GPI-80, a beta2-integrin-associated glycosylphosphatidylinositol-anchored protein, requires neutrophil differentiation with dimethyl sulfoxide in HL-60 cells.
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pubmed:affiliation |
Department of Immunology and Parasitology, Yamagata University School of Medicine, Yamagata-City, 990-9585, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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