| pubmed-article:12700652 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12700652 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:12700652 | lifeskim:mentions | umls-concept:C0376358 | lld:lifeskim |
| pubmed-article:12700652 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:12700652 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:12700652 | lifeskim:mentions | umls-concept:C0007745 | lld:lifeskim |
| pubmed-article:12700652 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:12700652 | lifeskim:mentions | umls-concept:C1517600 | lld:lifeskim |
| pubmed-article:12700652 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12700652 | pubmed:dateCreated | 2003-4-17 | lld:pubmed |
| pubmed-article:12700652 | pubmed:abstractText | The sphingomyelin metabolites ceramide and sphingosine are mediators of cell death induced by gamma-irradiation. We studied the production of ceramide and the effects of exogenous ceramide on apoptosis in LNCaP prostate cancer cells that are highly resistant to gamma-irradiation-induced cell death. LNCaP cells can be sensitized to gamma-irradiation by tumor necrosis factor alpha (TNF-alpha) and, to a lesser degree, by the agonistic FAS antibody CH-11. TNF-alpha activated intrinsic and extrinsic apoptosis pathways and increased ceramide and sphingosine levels in irradiated LNCaP cells. CH-11 activated only the extrinsic apoptosis pathways and had a negligible effect on ceramide and sphingosine levels in irradiated LNCaP cells. Exogenous ceramide and bacterial sphingomyelinase sensitized LNCaP cells to radiation-induced apoptosis and had a synergistic effect on cell death after irradiation with TNF-alpha, but not with CH-11. Cell death effects after exposure to ceramide and irradiation were blocked by the serine protease inhibitor TLCK (Na-p-tosyl-L-lysine-chloromethylketone), but not by the caspase inhibitor z-VAD (2-val-Ala-Asp(oMe)-CH(2)F). During LNCaP cell apoptosis induced by exogenous ceramide, we observed activation of caspase-9, but not caspases-8, -3, or -7. The effect of ceramide occurred largely via the intrinsic mitochondrial apoptosis pathway and enhanced TNF-alpha, but not CH-11 effects on irradiated cells. The data show that ceramide enhanced activation of the intrinsic apoptotic pathway and enhanced cell death induced by TNF-alpha with or without gamma-irradiation. TNF-alpha and gamma-irradiation elevated levels of endogenous ceramide and activated the intrinsic cell death pathway. | lld:pubmed |
| pubmed-article:12700652 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12700652 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12700652 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700652 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12700652 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12700652 | pubmed:issn | 1350-9047 | lld:pubmed |
| pubmed-article:12700652 | pubmed:author | pubmed-author:KimuraKK | lld:pubmed |
| pubmed-article:12700652 | pubmed:author | pubmed-author:SpiegelSS | lld:pubmed |
| pubmed-article:12700652 | pubmed:author | pubmed-author:GelmannE PEP | lld:pubmed |
| pubmed-article:12700652 | pubmed:author | pubmed-author:MarkowskiMM | lld:pubmed |
| pubmed-article:12700652 | pubmed:author | pubmed-author:EdsallL CLC | lld:pubmed |
| pubmed-article:12700652 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12700652 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:12700652 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12700652 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12700652 | pubmed:pagination | 240-8 | lld:pubmed |
| pubmed-article:12700652 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:12700652 | pubmed:meshHeading | pubmed-meshheading:12700652... | lld:pubmed |
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| pubmed-article:12700652 | pubmed:meshHeading | pubmed-meshheading:12700652... | lld:pubmed |
| pubmed-article:12700652 | pubmed:meshHeading | pubmed-meshheading:12700652... | lld:pubmed |
| pubmed-article:12700652 | pubmed:meshHeading | pubmed-meshheading:12700652... | lld:pubmed |
| pubmed-article:12700652 | pubmed:meshHeading | pubmed-meshheading:12700652... | lld:pubmed |
| pubmed-article:12700652 | pubmed:meshHeading | pubmed-meshheading:12700652... | lld:pubmed |
| pubmed-article:12700652 | pubmed:meshHeading | pubmed-meshheading:12700652... | lld:pubmed |
| pubmed-article:12700652 | pubmed:meshHeading | pubmed-meshheading:12700652... | lld:pubmed |
| pubmed-article:12700652 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12700652 | pubmed:articleTitle | Role of ceramide in mediating apoptosis of irradiated LNCaP prostate cancer cells. | lld:pubmed |
| pubmed-article:12700652 | pubmed:affiliation | Department of Oncology, Georgetown University, Washington, DC 20007-2197, USA. | lld:pubmed |
| pubmed-article:12700652 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12700652 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12700652 | lld:pubmed |
