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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 10
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pubmed:dateCreated |
2003-4-14
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pubmed:abstractText |
Lysophosphatidic acid (LPA) is a serum-borne phospholipid that exerts a pleiotropic range of effects on cells through activation of three closely related G-protein-coupled receptors termed LPA1/EDG-2, LPA2/EDG-4 and LPA3/EDG-7. Of these receptors, the LPA1 receptor is the most widely expressed. In this study, we investigated the agonist-induced endocytosis of the human LPA1 receptor, bearing an N-terminal FLAG epitope tag, in stably transfected HeLa cells. Treatment with LPA induced the rapid endocytosis of approximately 40% of surface LPA1 within 15 minutes. Internalization was both dose dependent and LPA specific since neither lysophophatidylcholine nor sphingosine-1-phosphate induced LPA1 endocytosis. Removal of agonist following 30 minutes incubation resulted in recycling of LPA1 back to the cell surface. LPA1 internalization was strongly inhibited by dominant-inhibitory mutants of both dynamin2 (K44A) and Rab5a (S34N). In addition, both dynamin2 K44A and Rab5 S34N mildly inhibited LPA1-dependent activation of serum response factor. Finally, our results also indicate that LPA1 exhibits basal, LPA-dependent internalization in the presence of serum-containing medium.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Dynamin II,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lysophosphatidic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Response Factor,
http://linkedlifedata.com/resource/pubmed/chemical/rab5 GTP-Binding Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9533
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1969-80
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12668728-DNA,
pubmed-meshheading:12668728-Dose-Response Relationship, Drug,
pubmed-meshheading:12668728-Dynamin II,
pubmed-meshheading:12668728-Endocytosis,
pubmed-meshheading:12668728-Epitopes,
pubmed-meshheading:12668728-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:12668728-HeLa Cells,
pubmed-meshheading:12668728-Humans,
pubmed-meshheading:12668728-Immunoblotting,
pubmed-meshheading:12668728-Lysophospholipids,
pubmed-meshheading:12668728-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:12668728-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:12668728-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12668728-Protein Structure, Tertiary,
pubmed-meshheading:12668728-Receptors, G-Protein-Coupled,
pubmed-meshheading:12668728-Receptors, Lysophosphatidic Acid,
pubmed-meshheading:12668728-Serum Response Factor,
pubmed-meshheading:12668728-Time Factors,
pubmed-meshheading:12668728-Transfection,
pubmed-meshheading:12668728-rab5 GTP-Binding Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Agonist-induced endocytosis of lysophosphatidic acid-coupled LPA1/EDG-2 receptors via a dynamin2- and Rab5-dependent pathway.
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pubmed:affiliation |
School of Biology and Petit Institute for Biosciences and Bioengineering, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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