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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2003-3-17
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pubmed:abstractText |
Selective inhibition of neuronal and inducible nitric oxide synthase (NOS) with 2-iminobiotin previously showed a reduction in brain cell injury. In the present study, we investigated the effects of 2-iminobiotin treatment on insulin-like growth factor-1 (IGF-1) expression, caspase activity and cytokine expression in a newborn piglet model of perinatal hypoxia-ischaemia. Newborn piglets were subjected to 1 h of hypoxia-ischaemia and were treated intravenously with vehicle or 2-iminobiotin. Vehicle-treated piglets showed reduced IGF-1 mRNA expression and increased caspase-3 activity and DNA fragmentation. 2-Iminobiotin treatment, administered immediately upon reperfusion, prevented these observations. No differences in caspase-8 and -9 activity and cytokine [interleukin (IL)-1alpha/beta, IL-6, tumour necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta] mRNA expression were demonstrated between vehicle- and 2-iminobiotin-treated piglets at 24 h following hypoxia-ischaemia. IGF-1 mRNA correlated inversely with caspase-3 and transferase-mediated dUTP-biotin in situ nick end labelling score in the cortex, but positively with caspase-8. Cytokine mRNA did not correlate with IGF-1 mRNA, caspase-3 activity or DNA fragmentation. The present results indicate that the previously demonstrated neuroprotective effect of 2-iminobiotin treatment after perinatal hypoxia-ischaemia coincided with a preservation of the endogenous IGF-1 production and reduced caspase-3 activity, but not with a significant decrease in cytokine production.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-iminobiotin,
http://linkedlifedata.com/resource/pubmed/chemical/Biotin,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:issn |
0378-5866
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pubmed:author |
pubmed-author:BlomgrenKlasK,
pubmed-author:GroenendaalFlorisF,
pubmed-author:HagbergHenrikH,
pubmed-author:HamersNicoleN,
pubmed-author:HeijnenCobiC,
pubmed-author:KosterJohannaJ,
pubmed-author:Peeters-ScholteCachaC,
pubmed-author:ZhuChanglianC,
pubmed-author:van BelFrankF,
pubmed-author:van Buul-OffersSylviaS,
pubmed-author:van den TweelEvelynE
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pubmed:copyrightInfo |
Copyright 2002 S. Karger AG, Basel
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
396-404
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:12640178-Animals,
pubmed-meshheading:12640178-Animals, Newborn,
pubmed-meshheading:12640178-Biotin,
pubmed-meshheading:12640178-Brain,
pubmed-meshheading:12640178-Caspases,
pubmed-meshheading:12640178-Cytokines,
pubmed-meshheading:12640178-DNA Fragmentation,
pubmed-meshheading:12640178-Enzyme Activation,
pubmed-meshheading:12640178-Enzyme Inhibitors,
pubmed-meshheading:12640178-Hypoxia-Ischemia, Brain,
pubmed-meshheading:12640178-In Situ Nick-End Labeling,
pubmed-meshheading:12640178-Insulin-Like Growth Factor I,
pubmed-meshheading:12640178-Models, Animal,
pubmed-meshheading:12640178-Nitric Oxide Synthase,
pubmed-meshheading:12640178-Nitric Oxide Synthase Type I,
pubmed-meshheading:12640178-Nitric Oxide Synthase Type II,
pubmed-meshheading:12640178-Primed In Situ Labeling,
pubmed-meshheading:12640178-RNA, Messenger,
pubmed-meshheading:12640178-Swine
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pubmed:year |
2002
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pubmed:articleTitle |
Effects of selective nitric oxide synthase inhibition on IGF-1, caspases and cytokines in a newborn piglet model of perinatal hypoxia-ischaemia.
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pubmed:affiliation |
Department of Neonatology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article
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