Linked Life Data

12560063

Source:http://linkedlifedata.com/resource/pubmed/id/12560063

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-1-31
pubmed:abstractText
A rapid and sensitive column-switching semi-micro high-performance liquid chromatography method was developed for the direct analysis of tiropramide in human plasma. The plasma sample (100 microl) was directly injected onto Capcell Pak MF Ph-1 precolumn where deproteinization and analyte fractionation occurred. Tiropramide was then eluted into an enrichment column (Capcell Pak UG C(18)) using acetonitrile-potassium phosphate (pH 7.0, 50 mM) (12:88, v/v) and was analyzed on a semi-micro C(18) analytical column using acetonitrile-potassium phosphate (pH 7.0, 10 mM) (50:50, v/v). The method showed excellent sensitivity (limit of quantification 5 ng/ml), and good precision (C.V.<or=5.9%), and speed (total analysis time 20 min). The calibration curve was linear in the concentration range 5-200 ng/ml (coefficient of determination equal to 0.998). This method was successfully applied to the pharmacokinetic study of tiropramide in volunteers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
185-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Semi-micro high-performance liquid chromatographic analysis of tiropramide in human plasma using column-switching.
pubmed:affiliation
College of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Shinyongdong, Iksan 570-749, South Korea.
pubmed:publicationType
Journal Article, Clinical Trial, Research Support, Non-U.S. Gov't