Linked Life Data

12549122

Source:http://linkedlifedata.com/resource/pubmed/id/12549122

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-1-28
pubmed:abstractText
This paper describes the DNA damage by hydrogen peroxide in HL-60 cells and the detection of 8-hydroxyguanine produced. The number of cells was at least about 2 x 10(7)/sample. Treatment of HL-60 cells with 0.4 mmol/L hydrogen peroxide for 24 h would result in DNA damage. The DNA samples were isolated, then qualitatively and quantitatively analyzed before hydrolysis. The results of experiments showed that 100-300 microL 98% formic acid could hydrolyze about 0.12 mg DNA samples completely in 150 degrees C for 1 h. After lyophilizing the DNA samples for 6-8 h, one DNA sample was trimethylsilylated completely with 150 microL BSTFA in 150 degrees C for 1 h. The recovery of trimethylsilylated 8-hydroxyguanine was 81.70%. The trimethylsilylated 8-hydroxyguanine was identified by GC/FID and CGC/MS-SIM parallely.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1000-8713
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
262-4
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
[Detection of 8-hydroxyguanine in the DNA of the H2O2-treated HL-60 cells by GC/FID].
pubmed:affiliation
Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang, 524023.
pubmed:publicationType
Journal Article, English Abstract