Source:http://linkedlifedata.com/resource/pubmed/id/12515911
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2003-1-7
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| pubmed:abstractText |
Two factors were found in the condition medium of neonatal pig liver fragments, which were capable of stimulating DNA synthesis in primary hepatocytes. They were named hepatocyte proliferation factor (HPF)-1 and HPF-2 and purified 1,025- and 2,580-fold, respectively. Both HPF-1 and HPF-2 seem to be anionic at pH 8.0 judged from the elution pattern of DEAE (DE52) column chromatography. HPF-1 was recovered as a non-adsorbed fraction in blue Sepharose and heparin Sepharose columns, and had a molecular weight of 26-31 kDa as estimated by gel filtration in high salt condition. Purified HPF-1 stimulated DNA synthesis of primary rat hepatocytes, but suppressed that of HepG2 cells. HPF-2 strongly bound to blue Sepharose and heparin Sepharose columns, and had a molecular weight of 71-90 kDa as estimated by SDS-PAGE under non-reduced condition. Purified HPF-2 stimulated DNA synthesis of primary rat hepatocytes dose dependently but did not suppress that of HepG2 cells. From further biological and chemical characteristics studied in this paper, HPF-1 and HPF-2 may be novel stimulating proteins for hepatocyte proliferation, although the possibility that they are already known growth factors can not be excluded without complete purification and its cloning.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
0951-6433
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1-14
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12515911-Animals,
pubmed-meshheading:12515911-Animals, Newborn,
pubmed-meshheading:12515911-Carcinoma, Hepatocellular,
pubmed-meshheading:12515911-Cells, Cultured,
pubmed-meshheading:12515911-Chromatography, Gel,
pubmed-meshheading:12515911-DNA,
pubmed-meshheading:12515911-Drug Stability,
pubmed-meshheading:12515911-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12515911-Growth Substances,
pubmed-meshheading:12515911-Hepatocytes,
pubmed-meshheading:12515911-Hot Temperature,
pubmed-meshheading:12515911-Humans,
pubmed-meshheading:12515911-Hydrogen-Ion Concentration,
pubmed-meshheading:12515911-Liver,
pubmed-meshheading:12515911-Liver Neoplasms,
pubmed-meshheading:12515911-Male,
pubmed-meshheading:12515911-Molecular Weight,
pubmed-meshheading:12515911-Rats,
pubmed-meshheading:12515911-Rats, Wistar,
pubmed-meshheading:12515911-Swine,
pubmed-meshheading:12515911-Tumor Cells, Cultured
|
| pubmed:year |
2002
|
| pubmed:articleTitle |
Hepatocyte proliferation factors from neonatal pig liver: purification and characterization.
|
| pubmed:affiliation |
Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, 259-1193 Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|