|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0010423,
umls-concept:C0033684,
umls-concept:C0332255,
umls-concept:C0439611,
umls-concept:C0439849,
umls-concept:C0445223,
umls-concept:C0521119,
umls-concept:C0728938,
umls-concept:C0936012,
umls-concept:C1412467,
umls-concept:C1552599,
umls-concept:C1704787,
umls-concept:C1709634
|
|
pubmed:issue |
Pt 1
|
|
pubmed:dateCreated |
2002-12-24
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Crystals of a partial extracellular fragment of a human sperm membrane protein YWK-II/APPH have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 A resolution at 100 K using Cu K(alpha) radiation. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 46.009, b = 67.387, c = 149.241 A, alpha = beta = gamma = 90 degrees. The presence of two molecules per asymmetric unit gives a crystal Volume per protein mass (V(M)) of 3.51 A(3) Da(-1) and a solvent content of 64.6% by Volume. A full set of X-ray diffraction data were collected to 2.8 A resolution from the native crystal.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0907-4449
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
59
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
146-9
|
|
pubmed:dateRevised |
2007-7-24
|
|
pubmed:meshHeading |
pubmed-meshheading:12499552-Amino Acid Sequence,
pubmed-meshheading:12499552-Amyloid beta-Protein Precursor,
pubmed-meshheading:12499552-Cell Membrane,
pubmed-meshheading:12499552-Conserved Sequence,
pubmed-meshheading:12499552-Crystallization,
pubmed-meshheading:12499552-Crystallography, X-Ray,
pubmed-meshheading:12499552-Humans,
pubmed-meshheading:12499552-Male,
pubmed-meshheading:12499552-Membrane Proteins,
pubmed-meshheading:12499552-Molecular Sequence Data,
pubmed-meshheading:12499552-Nerve Tissue Proteins,
pubmed-meshheading:12499552-Peptide Fragments,
pubmed-meshheading:12499552-Spermatozoa
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Crystallization and preliminary crystallographic analysis of a partial extracellular fragment of a sperm membrane protein YWK-II/APPH related to the Alzheimer betaA4-amyloid precursor protein.
|
|
pubmed:affiliation |
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, People's Republic of China.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
