| pubmed-article:12459191 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12459191 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:12459191 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:12459191 | lifeskim:mentions | umls-concept:C0596030 | lld:lifeskim |
| pubmed-article:12459191 | lifeskim:mentions | umls-concept:C0030580 | lld:lifeskim |
| pubmed-article:12459191 | lifeskim:mentions | umls-concept:C0015283 | lld:lifeskim |
| pubmed-article:12459191 | lifeskim:mentions | umls-concept:C0031670 | lld:lifeskim |
| pubmed-article:12459191 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:12459191 | pubmed:dateCreated | 2002-12-2 | lld:pubmed |
| pubmed-article:12459191 | pubmed:abstractText | The activation of beta-adrenergic receptors in rat parotid acinar cells causes intracellular cAMP elevation and appreciably stimulates the exocytotic release of amylase into saliva. The activation of Ca(2+)-mobilizing receptors also induces some exocytosis. We investigated the role of phospholipase D (PLD) in regulated exocytosis in rat parotid acinar cells. A transphosphatidylation assay detected GTPgammaS (a nonhydrolyzable analogue of GTP)-dependent PLD activity in lysates of rat parotid acinar cells, suggesting that PLD is activated by small molecular mass GTP-binding proteins. The PLD inhibitor, neomycin, suppressed cAMP-dependent exocytosis in saponin-permeabilized cells. Signaling downstream of PLD was disrupted by 1-butanol due to conversion of the PLD reaction product (phosphatidic acid) to phosphatidylbutanol. The stimulation of exocytosis by isoproterenol as well as by a Ca(2+)-mobilizing agonist (methacholine) was inhibited by 1-butanol. These results suggest that PLD is important for regulated exocytosis in rat parotid acinar cells. | lld:pubmed |
| pubmed-article:12459191 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12459191 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12459191 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12459191 | pubmed:author | pubmed-author:DohkeYokoY | lld:pubmed |
| pubmed-article:12459191 | pubmed:author | pubmed-author:FuruyamaShuns... | lld:pubmed |
| pubmed-article:12459191 | pubmed:author | pubmed-author:SugiyaHiroshi... | lld:pubmed |
| pubmed-article:12459191 | pubmed:author | pubmed-author:Fujita-Yoshig... | lld:pubmed |
| pubmed-article:12459191 | pubmed:author | pubmed-author:Hara-Yokoyama... | lld:pubmed |
| pubmed-article:12459191 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12459191 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12459191 | pubmed:pagination | 663-8 | lld:pubmed |
| pubmed-article:12459191 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12459191 | pubmed:articleTitle | Involvement of phospholipase D in the cAMP-regulated exocytosis of rat parotid acinar cells. | lld:pubmed |
| pubmed-article:12459191 | pubmed:affiliation | Department of Physiology, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakae-cho Nishi, Matsudo, Chiba 271-8587, Japan. | lld:pubmed |
| entrez-gene:25096 | entrezgene:pubmed | pubmed-article:12459191 | lld:entrezgene |
