Linked Life Data

12458798

Source:http://linkedlifedata.com/resource/pubmed/id/12458798

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2002-12-2
pubmed:abstractText
RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) induces the postranscriptional degradation of homologous transcripts. RNAi can be initiated by exposing cells to dsRNA either via transfection or endogenous expression. In mammalian systems, the sequence-specific RNAi effect has been observed by expression of 21-23 base transcripts capable of forming duplexes, or via expression of short hairpin RNAs. We describe here a facile PCR based strategy for rapid synthesis of siRNA expression units and their testing in mammalian cells. The siRNA expression constructs are constructed by PCR, and the PCR products are directly transfected into mammalian cells resulting in functional expression of siRNAs. This approach should prove useful for identification of optimal siRNA-target combinations and for multiplexing siRNA expression in mammalian cells.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11157775, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11201747, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11373684, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11498593, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11591334, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11641272, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11720281, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11910072, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11937629, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11972060, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11981564, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11981565, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-11981566, http://linkedlifedata.com/resource/pubmed/commentcorrection/12458798-12088155
pubmed:language
eng
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:author
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1454-60
pubmed:dateRevised
2009-11-18
pubmed:articleTitle
Functional siRNA expression from transfected PCR products.
pubmed:affiliation
Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA.