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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2002-11-8
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pubmed:abstractText |
Selenium (Se) is well known to be essential for cell culture when using a serum-free medium, but not when a medium containing serum is used. This finding suggests that serum contains some usable form of Se. To identify the Se-supplier, T-lymphoma (Jurkat) cells were cultured for 3 days in the presence of human serum immunodepleted of Se-containing serum protein, selenoprotein P or extracellular glutathione peroxidase. The Se-dependent enzyme activities (glutathione peroxidases and thioredoxin reductase) and Se content within the cells markedly decreased only when cultured with selenoprotein P-depleted serum. Compared with other Se-containing proteins, the addition of purified selenoprotein P to the selenoprotein P-depleted serum or a serum-free medium was the most effective for the recovery of cellular glutathione peroxidase activity (index of Se status). These results suggest that selenoprotein P functions as a Se-supply protein, delivering Se to the cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5746-51
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12423375-Cells, Cultured,
pubmed-meshheading:12423375-Cytosol,
pubmed-meshheading:12423375-DNA, Complementary,
pubmed-meshheading:12423375-Dose-Response Relationship, Drug,
pubmed-meshheading:12423375-Glutathione Peroxidase,
pubmed-meshheading:12423375-Glutathione Reductase,
pubmed-meshheading:12423375-Humans,
pubmed-meshheading:12423375-Jurkat Cells,
pubmed-meshheading:12423375-Proteins,
pubmed-meshheading:12423375-Selenium,
pubmed-meshheading:12423375-Selenoprotein P,
pubmed-meshheading:12423375-Selenoproteins,
pubmed-meshheading:12423375-Thioredoxin-Disulfide Reductase,
pubmed-meshheading:12423375-Time Factors
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pubmed:year |
2002
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pubmed:articleTitle |
Characterization of selenoprotein P as a selenium supply protein.
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pubmed:affiliation |
Department of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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