Source:http://linkedlifedata.com/resource/pubmed/id/12244208
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2002-9-23
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| pubmed:abstractText |
Immunoblots of a two-dimensional PAGE-separated HL-60 cell proteomic map and mass spectrometry were combined to characterize proteins targeted by autoantibodies produced by male (New Zealand White x BXSB)F(1) (WB) mice that develop lupus and anti-phospholipid syndrome. Analysis of sera sequentially obtained from seven individual mice at different ages showed that six proteins, vimentin, heat shock protein 60, UV excision-repair protein RAD23, alpha-enolase, heterogeneous nuclear ribonucleoprotein L, and nucleophosmin, were the targets of the B cell autoimmune response, and that autoantibodies to them were synthesized sequentially in an orderly pattern that recurred in all the male WB mice analyzed: anti-vimentin first and anti-nucleophosmin last, with anti-RAD23 and anti-heat shock protein 60, then anti-alpha-enolase and anti-heterogeneous nuclear ribonucleoprotein L Abs occuring concomitantly. Anti-vimentin reactivity always appeared before anti-cardiolipin and anti-DNA Abs, suggesting that vimentin is the immunogen initiating the autoimmune process. The pattern of HL-60 proteins recognized by female WB sera differed from that of male sera, indicating that the Y chromosome-linked autoimmune acceleration gene is not an accelerator but a strong modifier of the autoimmune response. Thus, 1) combining two-dimensional PAGE and mass spectrometry constitutes a powerful tool to identify the set of Ags bound by autoantibodies present in a single serum and the whole autoantibody pattern of an autoimmune disease; 2) the diversification of the autoimmune response in male WB mice occurs in a predetermined pattern consistent with Ag spreading, and thus provides a useful model to further our understanding of the development of the autoantibody response in lupus.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
169
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4046-53
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12244208-Animals,
pubmed-meshheading:12244208-Antigen-Antibody Reactions,
pubmed-meshheading:12244208-Autoantibodies,
pubmed-meshheading:12244208-Autoantigens,
pubmed-meshheading:12244208-Binding Sites, Antibody,
pubmed-meshheading:12244208-Crosses, Genetic,
pubmed-meshheading:12244208-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:12244208-Female,
pubmed-meshheading:12244208-HL-60 Cells,
pubmed-meshheading:12244208-Humans,
pubmed-meshheading:12244208-Immune Sera,
pubmed-meshheading:12244208-Lupus Erythematosus, Systemic,
pubmed-meshheading:12244208-Male,
pubmed-meshheading:12244208-Mice,
pubmed-meshheading:12244208-Mice, Inbred NZB,
pubmed-meshheading:12244208-Neoplasm Proteins,
pubmed-meshheading:12244208-Sex Characteristics,
pubmed-meshheading:12244208-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:12244208-Time Factors
|
| pubmed:year |
2002
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| pubmed:articleTitle |
Orderly pattern of development of the autoantibody response in (New Zealand White x BXSB)F1 lupus mice: characterization of target antigens and antigen spreading by two-dimensional gel electrophoresis and mass spectrometry.
|
| pubmed:affiliation |
Institut de la Santé et de la Recherche Médicale Unité 519, Faculté de Médecine et de Pharmacie, Hôpital Charles-Nicolle, Rouen, France.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|